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MOLECULAR CHARACTERIZATION OF BREAD WHEAT GENOTYPES TRITICUM AESTIVUM L. THROUGH MICROSATELLITES SSR MARKERS
Abstract
The molecular characterization of Genetic diversity of ten bread wheat (Triticum aestivum L.) varieties was evaluated at the DNA level using 6 agronomic characters and 16 wheat microsatellites molecular markers (Simple Sequence Repeat-SSR). SSR bands were scored across all genotypes, for presence (1) or absence (0) and transformed into 0/1 binary matrix. A pair-wise similarity matrix was generated with the software NTSYS. The Polymorphism Information Content (PIC) ranged from 0.23 to 0.70 respectively for the primer WMC 21 and WMC 50 with an average of 0.49 and 0.52 per primer pair. The similarity coefficient between cultivars ranged from 0.28 and 0.91 with an average of 0.60.Most of the genotypes showed a high degree of genetic similarity. The highest genetic distance value of 0.91 has been scored between Kauz/Pastor/Fiscal and Prl/2*Pastor and between Kauz/Pastor/Fiscal and Wbll1*2/Brambling. The lowest genetic distance value of 0.28 has been scored between Arz and Babax/ Lr42// Babax*2/3/Kukuna. Cluster analysis based on microsatellite allelic diversity discriminated the varieties into different clusters. The morpho-agronomical variability was analyzed using the Principal Component Analysis. The results demonstrate the utility of microsatellite markers for detecting polymorphism to estimate genetic diversity.
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