Scholarly record
IMPROVING THE STAINING PERFORMANCE OF A NOVEL LUMINESCENT DYE FOR ENHANCED MUSCLE IMAGING IN BIOLOGICAL SPECIMENS
Abstract
Polycyclic aromatic dyes, are widely used as fluorescent labels, and of super-resolution microscopy. Dyes exhibit high photo stability, broad emission spectra making them suitable for advanced bioimaging. That is especially important in parasitology, where fluorescent labelling enables detailed investigation of pathogen morphology, and dynamic biological processes in living systems. In previous studies, ethanol was identified as an effective fixation agent because of its low toxicity and ability to preserve tissue structure. It was also used as a solvent for luminescent dyes. However, some dyes dissolved in ethanol did not provide sufficient visualization of muscle structures. The newly synthesized fluorescent dye EA53 was dissolved in ethanol and chloroform. Staining patterns was analyzed using CLS microscopy. Chloroform enhanced the luminescent properties of dye. While internal structures showed no significant differences, visualization of external structures improved, and muscle fibers longitudinal, diagonal, and circular became more distinct that contributing to the optimization of staining protocols for biological imaging.
Publication details
ReferencesPending
Structured references will appear here after the reference import pass. The count is preserved now so the scholarly record is not incomplete.