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USE OF BIOLOG SYSTEM FOR AUTOCHTONOUS MICROBIOTA IN CONTAMINATED SOIL
Abstract
Microorganisms are ubiquitous in nature and possess unique biochemical possibilities to assimilate a vast array of available substrates and produce unique se metabolit and biotransformation pathways. Long-term exposure to elevated arsenic and antimony concentrations has led to the selection of specifically resistant autochthonous microorganisms. In the cultivable component of isolated bacterial microorganisms, Bacillus was identified as the dominant genus, which has been represented by nine species. Interesting phenotypic properties have been confirmed in isolated species, which have been tested the Biolog system. The Biolog GEN III MicroPlateTM, based on the company's Phenotype Array Technology, was recently introduced for rapid identification and characterization of microorganisms (Biolog Inc., USA). The biochemical characterization of isolated bacteria leading to their successful identification is important for evaluating differences between individual bacterial strains. The high incidence of Bacillus species affects the mobility of the metals and semi-metals present, the diversity of their metabolic pathways promotes the gradual reduction of metals in soil contaminated by mining and industrial activities.
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