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STAINING OF ECONOMICALLY IMPORTANT PARASITIC NEMATODES BY DEVELOPED DERIVATIVES OF BENZANTHRONE LUMINOPHORE

Inese Gavarāne

First published: 2018-06-20https://doi.org/10.5593/sgem2018/6.2/s25.077View metrics

Abstract

Nematodes belonging to the genus Trichinella are zoonotic parasites with a high impact on the animal trade and human health. Foodborne pathogens of Trichinella genus are known as causative agents of human trichinellosis, a disease that is a public health hazard. According to the Commission Regulation (EU) No. 2015/1375 all animals, which are potential carriers of Trichinella spp. larvae and are for human consumption, must be tested according to one of the approved tests. For assessment of developed benzanthrone luminophore P13 the Trichinella britovi larvae samples were selected. Confocal laser scanning fluorescence microscopy was applied to visualize stained larvae. Developed technique is appropriate and demonstrated sufficient results for samples preserved in 96% ethanol for up to three years long period and for larvae obtained from two years frozen muscle samples. Staining of internal organs by developed technique may be an invaluable source of data for studies of parasite structure and variety.

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Publication details

Title
STAINING OF ECONOMICALLY IMPORTANT PARASITIC NEMATODES BY DEVELOPED DERIVATIVES OF BENZANTHRONE LUMINOPHORE
Authors
Inese Gavarāne
Proceedings
SGEM International Multidisciplinary Scientific GeoConference EXPO Proceedings; 18th International Multidisciplinary Scientific GeoConference SGEM2018, Nano, Bio and Green - Technologies for a Sustainable Future
Publisher
STEF92 Technology
Year
2018
Pages
581-588
SWS Citekey
Gavarane201825581588
ISSN
1314-2704
ISBN
978-619-7408-51-5
Language
en
Publication type
Conference Paper
Proceedings contents
Open official contents
Keywords
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