Scholarly record
OXIDATIVE DAMAGE IN ZEBRAFISH EXPOSED TO ENVIRONMENT REALISTIC CONCENTRATIONS OF ROUNDUP AND CHLORPYRIFOS
Abstract
The rapid development of agricultural sector is becoming the main factor leading to continuously increasing of specific pollution of surface water. The aim of this study was to assess the selected parameters of oxidative stress in zebrafish (Danio rerio) after their exposure to environmental realistic concentrations of roundup and chlorpyrifos during 14 days. Our results confirm that roundup and chlorpyrifos in the environmentally relevant concentrations promote oxidative stress in zebrafish liver. In particular, roundup in subchronic concentration increased glutathione level, glutathione reductase and catalase activities, but didn’t provoke reactive oxygen species overflow. However, no significant changes were registered when fish were treated with roundup in higher studied concentration (500 ?g L?1). On the other hand, chlorpyrifos caused clear oxidative damage was registered as an increase in reactive oxygen species and protein carbonyls in parallel with prominent oppression of catalase activity. In general, therefore, it seems that chlorpyrifos is more toxic to zebrafish than roundup based on calculation of the integrate biomarker index. The toxicity of studied pesticides could be ranked as chlorpyrifos (0.1 ?g L?1) ? chlorpyrifos (3.0 ?g L?1) > roundup (500 ?g L?1) > roundup (15 ?g L?1). In sum, constantly growing agrochemical pollution might be of great concern and definitely can affect biodiversity in natural environment. The catalase activity and protein carbonyls concentration in zebrafish were identified as useful biomarkers of oxidative stress that warrants their application in the field investigations.
Publication Impact Profile
- Captures
- Mendeley - Readers: 2
Publication details
References15
Relyea R. A. The impact of insecticides and herbicides on the biodiversity and productivity of aquatic communities, Ecol. Appl., vol. 15, pp 618-627, 2005.
De A., Bose R., Kumar A. & Mozumdar S. Worldwide pesticide use, In: Targeted Delivery of Pesticides Using Biodegradable Polymeric Nanoparticles, Springer, New Delhi, 2014.
Ye Z., Wu F. & Hennessy D. A. Environmental and economic concerns surrounding restrictions on glyphosate use in corn, PNAS USA, vol. 118 (18): e2017470118, 2021. DOI: 10.1073/pnas.2017470118.
Hanke I., Singer H. & Hollender J. Ultratrace-level determination of glyphosate, aminomethylphosphonic acid and glufosinate in natural waters by solid-phase extraction followed by liquid chromatography-tandem mass spectrometry: performance tuning of derivatization, enrichment and detection, Anal. Bioanal. Chem., vol. 391, pp 2265-2276, 2008.
Byer J.D., Struger J., Klawunn P., Todd A. & Sverko E. Low cost monitoring of glyphosate in surface waters using the ELISA method: An evaluation, Environ. Sci. Technol., vol. 42 (16), pp 6052-6057, 2008.
Shaffo F.C., Grodzki A.C., Fryer A.D. & Lein P.J. Mechanisms of organophosphorus pesticide toxicity in the context of airway hyperreactivity and asthma, Am. J. Physiol. Lung. Cell Mol. Physiol., vol. 315 (4), pp 485-501, 2018.
Cavalcante D., Martinez C. & Sofia S. Genotoxic effects of Roundup® on the fish Prochilodus lineatus, Mutat Res/Genet. Toxicol. Environ. Mutagen., vol. 655 (1), pp 41-46, 2008.
Modesto K.A. & Martinez C.B. Roundup causes oxidative stress in liver and inhibits acetylcholinesterase in muscle and brain of the fish Prochilodus lineatus, Chemosphere, vol. 78 (3), pp 294-299, 2010.
Bodnar O., Horyn O., Khatib I. & Falfushynska H. Multibiomarker assessment in zebrafish Danio rerio after the effects of malathion and chlorpyrifos. Toxicol. Environ. Health Sci., 2021. In press.
Bartoskova M., Dobsikova R., Stancova V., Zivna D., Blahova J., Marsalek P., Zelnickova L., Bartos M., Di Tocco F.C. & Faggio C. Evaluation of ibuprofen toxicity for zebrafish (Danio rerio) targeting on selected biomarkers of oxidative stress, Neuro Enocrin. Let., vol. 34, pp 102-108, 2013.
Henao E., Murphy P.J., Falfushynska H., Horyn O., Evans D.M., Klimaszyk P. & Rzymski P., Polymethoxy-1-Alkenes screening of Chlorella and Spirulina food supplements coupled with in vivo toxicity studies, Toxins, vol. 12 (2), 111, 2020.
Sinhorin V.D., Sinhorin A.P., Teixeira J.M., Mileski K.M., Hansen P.C., Moreira P.S., Kawashita N.H., Baviera A.M. & Loro V.L. Effects of the acute exposition to glyphosate-based herbicide on oxidative stress parameters and antioxidant responses in a hybrid Amazon fish surubim (Pseudoplatystoma sp.), Ecotoxicol. Environ. Saf., vol. 106, pp 181-187, 2014.
Ferreira D., Motta A.C., Kreutz L.C., Toni C., Loro V.L. & Barcellos L.J.G. Assessment of oxidative stress in Rhamdia quelen exposed to agrichemicals, Chemosphere, vol. 79 (9), pp 914-921, 2010.
Lushchak O.V., Kubrak O.I., Storey J.M., Storey K.B. & Lushchak V.I. Low toxic herbicide Roundup induces mild oxidative stress in goldfish tissues, Chemosphere, vol. 76 (7), pp 932-937, 2009.
Toledo-Ibarra G.A., Diaz Resendiz K.J., Ventura-Ramon G.H., Gonzalez-Jaime F., Vega-Lopez A., Becerril-Villanueva E., Pavon L. & Giron-Perez M.I. Oxidative damage in gills and liver in Nile tilapia (Oreochromis niloticus) exposed to diazinon, Comp. Biochem. Physiol. A Mol. Integr. Physiol., vol. 200, pp 3-8, 2016.
View or Download full articleAccess options
SWS access login
Login as SWS Scientific CommitteeLogin as SWS Scientific PartnerLogin as SWS AuthorAuthors and approved SWS contributors will read and export their own linked papers after identity matching by SWS profile, email and SGEM GlobalID.
For librarian assistance: [email protected]
Purchase Instant Access
- Article can be downloaded after successful payment.
- Article may be used according to SWS library access terms.
- Article cannot be redistributed.