Scholarly record
ROOTING IN ARTEMISIA ALBA TURRA AS A MODELLING CLUE IN CHLOROPLAST ARCHITECTURE AND CYTOKININ METABOLIC CONJUGATION IN VITRO
Abstract
Shoot cultures of the essential oil bearing plant Artemisia alba Turra (Asteraceae) were studied with the aim to elaborate the effect of in vitro rooting on cytokinin (CK) conjugation and chloroplast architecture in vitro. For this purpose, two in vitro morphotypes were induced ? (1) the root developing morphotype on plant growth regulator-free as well as on auxin indole-3-butyric acid (IBA)-containing media and (2) the root suppressed morphotype, obtained by a combination of IBA with CK N6-benzyladenine (BA). Endogenous CK levels were analysed by HPLC-ESI-MS/MS. The thylakoid membrane samples were prepared from 1-hour dark- and ice-cold adapted plants. Thylakoid imaging was done by a NanoScopeV system, Bruker Inc. atomic force microscope in a tapping mode in air. In all of the in vitro samples the overall levels of cis-zeatin-type CKs generally significantly exceeded those of trans-zeatin types ? the tendency being more pronounced for aerial samples, and especially for the aerials of the root suppressed morphotype. Further analysis showed that CK N- and O-glucoconjugation patterns were considerably affected by morphogenetic changes. Root suppression and especially callusogenesis were established as a factor causing a drop of N-glucosylation in both aerial and underground samples. In contrast, O-glucosylation was enhanced in both above- and underground tissues of the root suppressed plants. As N- and O-glucosylation represent respective deactivation and storage pathways in CK metabolism, the lack of root formation and enhanced callusogenesis seem to be associated with a reduced metabolic inactivation of CKs and/or increased formation of a CK ?reserve? pool. Marked alteration of plastid morphology was observed in samples with suppressed rooting. It is well known that biogenesis of trans-zeatin-type CKs in plant cells is spatially bound to plastids, while formation of cis-zeatin-type CKs is compartmented in the cytosol. The observed dependencies suggest an interplay between cis- and transzeatins and also among other particular CK forms in A. alba plants cultured in vitro via possible moderation of chloroplast structure.
Publication Impact Profile
Publication details
References7
Jakovljević M.R., Grujičić D., Živanović M., Stanković M., Ćirić A., Djurdjević P., Todorović Ž., Živančević-Simonović S., Mihaljević O., Milošević-Djordjević O., Ethyl acetate extracts of two Artemisia species: analyses of phenolic profile and anticancer activities against SW-480 colon cancer cells, Natural Product Communications, vol. 14, pp. 1–6, 2019.
Letham D.S., Cytokinins as phytohormones – sites of biosynthesis, translocation, and function of translocated cytokinin, in: Cytokinins: chemistry, activity, and function, Mok D.W.S. & Mok M.C., eds., USA, CRC Press New York, pp. 57-80, 1994.
Sakakibara H., Cytokinins: activity, biosynthesis, and translocation, Annual Review of Plant Biology, vol. 57, pp. 431-449, 2006.
Danova K., Motyka V., Todorova M., Trendafilova A., Krumova S., Dobrev P., Andreeva T., Oreshkova T., Taneva S., Evstatieva L., Effect of cytokinin and auxin treatments on morphogenesis, terpenoid biosynthesis, photosystem structural organization, and endogenous isoprenoid cytokinin profile in Artemisia alba Turra in vitro, Journal of Plant Growth Regulation, vol. 37, pp. 403-418, 2018.
Harrison M.A., Melis A., Organization and Stability of Polypeptides Associated with the Chlorophyll a-b Light-Harvesting Complex of Photosystem-II, Plant and Cell Physiology, vol. 33, pp. 627–637, 1992.
Dubreuil C., Jin X., de Dios Barajas-López J., Hewitt T.C. Tanz S.K. Dobrenel T., Schröder W.P., Hanson J., Pesquet E., Grönlund A., Small I., Strand A., Establishment of Photosynthesis through Chloroplast Development Is Controlled by Two Distinct Regulatory Phases, Plant Physiology, vol. 176, pp. 1199-1214, 2018.
Cortleven A., Schmülling T., Regulation of chloroplast development and function by cytokinin, Journal of Experimental Botany, vol. 66, pp. 4999–5013, 2015.
View or Download full articleAccess options
SWS access login
Login as SWS Scientific CommitteeLogin as SWS Scientific PartnerLogin as SWS AuthorAuthors and approved SWS contributors will read and export their own linked papers after identity matching by SWS profile, email and SGEM GlobalID.
For librarian assistance: [email protected]
Purchase Instant Access
- Article can be downloaded after successful payment.
- Article may be used according to SWS library access terms.
- Article cannot be redistributed.
