Scholarly record
ANTIMICROBIAL RESISTANCE AND BIOFILM FORMATION OF SOME STAPHYLOCOCCUS SPECIES ISOLATED FROM TRADITIONAL CHEESES
Abstract
This research aimed to determine the occurrence of different Staphylococcus spp., in three types of Romanian traditional cheeses made of raw milk, and to establish their antimicrobial resistance profile. Additionally, we have also screened the biofilm forming capacity of the S. aureus isolates. Thus, 25 samples (cow telemea n = 10, sheep telemea n = 5, and cow ca? n = 10) were collected from local small producers that sell in urban fresh markets in Timi? county. A total of 64% (16/25) of the examined samples were positive. The bacterial strains were isolated on Mannitol Salt Agar (MSA) medium, then Baird-Parker medium was used to emphasize the coagulase activity. Furthermore, using the Vitek 2 Compact equipment, 17 strains were identified and included in 4 species of Staphylococcus genus, respectively one coagulase-positive (CPS) represented by Staphylococcus aureus 52.97% (9/17), and 3 coagulase-negative (CNS) comprised of S. epidermidis 23.52% (4/17), S. chromogenes 11.76% (2/17) and S. saprophyticus 11.76% (2/17). Disc diffusion method showed that the S. aureus isolates presented more resistance towards penicillin (100%), erythromycin, oxacillin, gentamicin, tetracycline (66.66%), and ciprofloxacin, clindamycin (55.55%). CNS isolates were more resistant towards penicillin (83.33%), erythromycin (66.66%), oxacillin (50), and tetracyclin (41.66%). From the total of 9 S. aureus, all were able to produce biofilms with absorbance at 570 nm.
Publication Impact Profile
- Citations
- CrossRef - Citation Indexes: 1
- Scopus - Citation Indexes: 0
- Captures
- Mendeley - Readers: 2
Publication details
References10
Morar A., Ban-Cucerzan A., Herman V., Tirziu E., Sallam K. I., Abd-Elghany S. M., Imre K., Multidrug Resistant Coagulase-Positive Staphylococcus aureus and Their Enterotoxins Detection in Traditional Cheeses Marketed in Banat Region, Romania, Antibiotics (Basel, Switzerland), vol.10/issue12, 2021. DOI: 10.3390/antibiotics10121458
Hoveida L., Halaji M., Rostami S., Mobasherizadeh S., Biofilm-producing ability of Staphylococcus spp isolated from different foodstuff products. Annali di igiene : medicina preventiva e di comunita, col. 31/issue 2, pp. 140�147, 2019.
Vyrostkova J., Regecova I., Zigo F., Semjon B., Gregova G., Antimicrobial Resistance of Staphylococcus sp. isolated from Cheeses, Animals, vol.12/issue 1:36, 2021. DOI: 10.3390/ani12010036
Chajecka-Wierzchowska W., Zadernowska A., Gajewska J., S. epidermidis strains from artisanal cheese made from unpasteurized milk in Poland - Genetic characterization of antimicrobial resistance and virulence determinants, International journal of food microbiology, vol. 294, pp. 55�59, 2019. DOI: 10.1016/j.ijfoodmicro.2019.02.004
Filipello V., Di Ciccio P.A., Colagiorgi A., Tilola M., Romano A., Vitale N., Losio M.N., Luini M., Zanardi E., Ghidini S., Ianieri A., Finazzi G., Molecular characterisation and biofilm production in Staphylococcusaureus isolates from the dairy production chain in Northern Italy, International Dairy Journal, vol. 91, pp. 110-118, 2019. DOI: 10.1016/j.idairyj.2018.10.002
European Committee on Antimicrobial Susceptibility Testing (EUCAST), https://www.eucast.org/fileadmin/src/media/pdfs/eucast_files/breakpoint_tables/v_11.0 _breakpoint_tables.pdf.
O�Toole G.A., Microtiter dish biofilm formation assay, Journal of Visualized Experiments, vol. 47/issue 2437, 2011. DOI: 10.3791/2437-v
Stepanovic S., Vukovic D., Hola V., Bonaventura G.D., Djukic S, Cirkovic I., Ruzicka, F., Quantification of biofilm in microtiter plates: Overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci, APMIS: acta pathologica, microbiologica, et immunologica Scandinavica, vol. 115/issue 8, pp. 891-899, 2007. DOI: 10.1111/j.1600-0463.2007.apm_630.x
Bulajic S., Colovic S., Misic D., Djordjevic J., Savic-Radovanovic R., Asanin J., Ledina T., Enterotoxin production and antimicrobial susceptibility in staphylococci isolated from traditional raw milk cheeses in Serbia, Journal of Environmental Sciences and Health part B, vol. 52, pp. 864�870, 2017. DOI: 10.1080/03601234.2017.1361764
Chajecka-Wierzchowska W., Zadernowska A., Nalepa B., Sierpinska M., Laniewska-Trokenheim L, Coagulase-negative staphylococci (CoNS) isolated from ready-to-eat food of animal origin--phenotypic and genotypic antibiotic resistance, Food microbiology, vol. 46, pp. 222�226, 2015. DOI: 10.1016/j.fm.2014.08.001
View or Download full articleAccess options
SWS access login
Login as SWS Scientific CommitteeLogin as SWS Scientific PartnerLogin as SWS AuthorAuthors and approved SWS contributors will read and export their own linked papers after identity matching by SWS profile, email and SGEM GlobalID.
For librarian assistance: [email protected]
Purchase Instant Access
- Article can be downloaded after successful payment.
- Article may be used according to SWS library access terms.
- Article cannot be redistributed.