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THE REACTOR SYSTEM FOR BIOSYNTHESIS OF BACTERIAL CELLULOSE WITH DESIRED MICROFIBRIL ORIENTATION

Ksenia Bolotova

First published: 2018-06-20https://doi.org/10.5593/sgem2018/6.1/s24.067View metrics

Abstract

The drip feed reactor system for continuous biosynthesis of bacterial cellulose with desired microfibril orientation is proposed. Native bacterial cellulose is obtained on the glucose media using symbiotic complex of yeast and bacteria A.xylinum. Micromorphological characteristics of the bacterial cellulose structure are visualized using SEM Sigma VP ZEISS scanning electron microscopy. The crystallinity degree of the specimens is calculated by new method using X-ray diffractometry (XRD-7000S diffractometer). The 28 cm-long bacterial cellulose specimen was obtained in drip feed reactor system during 12 days continuous cultivation. Internal biofouling of reactor system tubes was observed. Globular microfibril structures were formed in the matrix of bacterial cellulose in the drip feed reactor system unlike the cellulose obtained in static conditions. The diffraction patterns of bacterial cellulose specimens synthesized in static and dynamic (the drip feed reactor system) conditions were obtained. It was shown that basic crystalline compound of the specimens was I?-type cellulose. The crystallinity degree of bacterial cellulose from the reactor system was 12 %, whereas for cellulose synthesized in static conditions this parameter was equal to 15 %. The structure of bacterial cellulose became less oriented because of increasing of ratio of amorphous parts in comparison with structure of bacterial cellulose synthesized in static conditions.

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Publication details

Title
THE REACTOR SYSTEM FOR BIOSYNTHESIS OF BACTERIAL CELLULOSE WITH DESIRED MICROFIBRIL ORIENTATION
Authors
Ksenia Bolotova
Proceedings
SGEM International Multidisciplinary Scientific GeoConference EXPO Proceedings; 18th International Multidisciplinary Scientific GeoConference SGEM2018, Nano, Bio and Green - Technologies for a Sustainable Future
Publisher
STEF92 Technology
Year
2018
Pages
505-510
SWS Citekey
Bolotova201824505510
ISSN
1314-2704
ISBN
978-619-7408-50-8
Language
en
Publication type
Conference Paper
Keywords
microfibril?s orientationbacterial celluloseelectron microscopycrystallinity degree
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