Scholarly record
THE REACTOR SYSTEM FOR BIOSYNTHESIS OF BACTERIAL CELLULOSE WITH DESIRED MICROFIBRIL ORIENTATION
Abstract
The drip feed reactor system for continuous biosynthesis of bacterial cellulose with desired microfibril orientation is proposed. Native bacterial cellulose is obtained on the glucose media using symbiotic complex of yeast and bacteria A.xylinum. Micromorphological characteristics of the bacterial cellulose structure are visualized using SEM Sigma VP ZEISS scanning electron microscopy. The crystallinity degree of the specimens is calculated by new method using X-ray diffractometry (XRD-7000S diffractometer). The 28 cm-long bacterial cellulose specimen was obtained in drip feed reactor system during 12 days continuous cultivation. Internal biofouling of reactor system tubes was observed. Globular microfibril structures were formed in the matrix of bacterial cellulose in the drip feed reactor system unlike the cellulose obtained in static conditions. The diffraction patterns of bacterial cellulose specimens synthesized in static and dynamic (the drip feed reactor system) conditions were obtained. It was shown that basic crystalline compound of the specimens was I?-type cellulose. The crystallinity degree of bacterial cellulose from the reactor system was 12 %, whereas for cellulose synthesized in static conditions this parameter was equal to 15 %. The structure of bacterial cellulose became less oriented because of increasing of ratio of amorphous parts in comparison with structure of bacterial cellulose synthesized in static conditions.
Publication Impact Profile
Publication details
References6
Lavrentyeva E.V., Radnagurueva A.A., Banzaraktsaeva T.G., Bazarov S.M., Barkhutova D.D., Ulzetueva I.D., Chernyavsky M.K., Kabilov M.R., Khakhinov V.V. Phylogenetic analysis of the microbial mat in the hot spring Garga (Baikal rift zone) and the diversity of natural peptidases, Vavilov Journal of Genetics and Breeding, Russia, vol. 21 (8), pp. 959-963, 2017.
Lin S.P., Loira Calvar I., Catchmark J.M., Liu Je-R., Demirci Al., Cheng K.-Ch., Biosynthesis, production and applications of bacterial cellulose, Cellulose, Netherlands, vol. 20/issue 5, pp 2191-2219, 2013.
Huang Y., Zhu C., Yang J., Nie Y., Chen C., Sun D. Recent advances in bacterial cellulose, Cellulose, Netherlands, vol. 21, pp. 1-30, 2014.
Chukhchin D.G., Malkov A.V., Tyshkunova I.V., Mayer L.V., Novozhilov E.V., Diffractometric method for determining the degree of crystallinity of materials, Crystallography Reports, Russia, vol. 61/issue 3, pp 371-375, 2016.
Bolotova K., Travina O., Chukhchin D., Novozhilov E., The morphological structure of microcrystallites derived from plant and bacterial cellulose, Conference proceedings 17 international multidisciplinary scientific geoconference SGEM 2017, Bulgaria, issue 61, pp. 407-412, 2017.
Bolotova K.S., Chukhchin D.G., Mayer L.V., Gur'yanova A.A., Morphological features of the fibrillar structure of plant and bacterial cellulose, Lesnoi zhurnal, Russia, vol. 6, pp 153-165, 2016.
View or Download full articleAccess options
SWS access login
Login as SWS Scientific CommitteeLogin as SWS Scientific PartnerLogin as SWS AuthorAuthors and approved SWS contributors will read and export their own linked papers after identity matching by SWS profile, email and SGEM GlobalID.
For librarian assistance: [email protected]
Purchase Instant Access
- Article can be downloaded after successful payment.
- Article may be used according to SWS library access terms.
- Article cannot be redistributed.
