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ASSESSMENT OF THE BIODEGRADABILITY OF CATIONIC FLOCCULANTS BY MICROORGANISMS ISOLATED FROM SEWAGE SLUDGE OBTAINED ON WASTEWATER TREATMENT PLANTS
Abstract
Annually, a city with 500,000 inhabitants generates approximately 115.5 million cubic meters of wastewater. The treatment of this wastewater is efficiently managed using activated sludge, which comprises a diverse community of protozoa, infusoria, lower crustaceans, algae, and microorganisms. These organisms facilitate the effective treatment of large volumes of wastewater in treatment plants. However, the activated sludge proliferates by consuming organic materials in the wastewater, resulting in the accumulation of substantial volumes of sewage sludge in these plants. To manage this sludge, it is pre-dewatered using flocculants. While flocculants are effective for dewatering, the presence of polyacrylamides poses a challenge due to their resistance to degradation and potential environmental toxicity. Therefore, before the biological treatment of sewage sludge, it is essential to purify it from flocculants. This study aimed to isolate bacteria capable of degrading cationic flocculants from the activated sludge of municipal wastewater treatment plants, screen their growth rates, identify the most promising microorganism species, and evaluate their ability to degrade cationic flocculants. Two bacterial isolates were obtained, capable of growing on a medium where the sole carbon source was Greenlife K-35 and Mainfloc 5858 brand flocculants. The most effective isolate was identified based on growth curves. DNA sequencing of the 16S rRNA region by the Sanger method identified this strain as Pseudomonas nitroreducens. In the presence of this strain, the degradation rates of Mainfloc 5858 and Greenlife K-35 were 61.5% and 59.6% at 28 days, respectively, as evaluated using the OxiTop system.
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