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RESEARCH AND EVALUATION OF RAT BLOOD INDICATORS AT REPEATED INHALATION EXPOSURE BY COPPER (II) OXIDE NANOPARTICLES AEROSOL
Abstract
The blood parameters of rats under repeated inhalation exposure to copper oxide (II) nanoparticles aerosol (CuO NPs) compared to the microdispersed analogue (CuO MPs) have been studied and evaluated. According to the results of the study of the size, specific surface area, and total volume of pores, it was found that the sample under test is a nanomaterial. Rats were exposed to aerosols of aqueous suspensions of CuO NPs and CuO MPs for 10 days at 4 h/day in an inhalation system with a full-body chamber. The actual concentration in the chamber of CuO NPs was 1.17 ± 0.18 mg/m3, CuO MPs -1.39 ± 0.21 mg/m3. Twenty-four hours after the last exposure, 3 cm3 of blood wasdrawn from the tail vein to study biochemical and hematological parameters. Changesin biochemical and hematological blood parameters of rats exposed to nanomaterial inthe form of an increase in the activity of alanine aminotransferase (ALT), aspartateaminotransferase up to 2.2 times were noted, alkaline phosphatase, gamma-glutamyltranspeptidase (GGT), lactate dehydrogenase, amylase, antioxidant activity, increase ofC-reactive protein, malondialdehyde concentrations up to 1.39 times and decrease ofurea content by 1.41 times; increasing up to 5.4 times in the relative number ofeosinophils and leukocytes, the red blood cell distribution width, decreasing up to 1.4times of segmented neutrophils and monocytes. Rats exposed to CuO MPs showedsimilar changes in the studied indices, except for an increase in AST, GGT, and adecrease in the proportion of segmented neutrophils established when exposed to CuONPs. At the same time, the revealed 1.3-2.4-fold increase in some biochemical andhematological blood parameters of rats under the action of CuO NPs compared to CuOMPs indicates a more pronounced toxic effect of the studied nanomaterial on organs andsystems.
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