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CREATION OF IN VITRO MODEL OF HUMAN BLOOD-BRAIN BARRIER, COMPLETELY IDENTIFICAL TO REAL BLOOD-BRAIN BARRIER
Abstract
The creation of in vitro model of human BBB, completely identical to the real BBB, was necessary for the possibility of studying the transport and penetration of drugs and xenobiotics and is still very popular and urgent task. In order to reproduce the most close to the real in vitro model of BBB, a microfluidic platform is used, which allows more carefully reproduce the structure of BBB, as well as physiological tangential stress on the surface of endothelial cells (0.3 - 2 PA). An innovative development was a new class of in vitro models: organs-on-chip, combining the advantages of both in vivo and in vitro models. These so-called chips are micro-fluid devices in which tissues are cultivated in special conditions that best reflect the real microenvironment of these tissues. In this paper, an analytical review of modern scientific and technical, regulatory, methodological literature, affecting the scientific and technical problem investigated in the framework of applied research. It is concluded that the BBB-on-chip models can be used to study complex biological processes, combine the advantages of in vitro and in vivo models, have high predictive power, and be a valuable research tool in addition to the classical in vitro and in vivo methods.
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References10
Blinov D.V., Current views on the role of the violation of the blood-brain barrier in the pathogenesis of central nervous system diseases. Part 1: Structure and formation of the blood-brain barrier, Epilepsy and paroxysmal states, Russia, №3, p. 65-67, 2013.
Morgun, A., Century. The main functions of the blood-brain barrier, Siberian Medical Journal (Irkutsk), Russia, V. 109, № 2, P. 5-8, 2012.
Senyavina N.V., Trushkin E.V., Rusanov A.L., et al., Modern technologies of in vitro testing: microbioreactor, Biotechnology, V. 1, P. 51–58, 2013.
Uwe M., Heike W., Silke H. et al. ‘human-on- a-chip’ developments: A translational cuttingedge alternative to systemic safety assessment and efficiency evaluation of substances in laboratory animals and man? Atlanta, vol. 40, P. 235–257, 2012.
Ilka W., Eva-Maria M., Sven B. et al., A dynamic multi-organ- chip for long-term cultivation and substance testing proven by 3d human liver and skin tissue co-culture, Lab Chip, Vol. 13, no. 18, P. 3538–3547, 2013.
Van der Helm M. W., Van der Meer A. D., Eijkel J. C. T., Van den Berg A., Segerink L. I., Microfluidic organ-on-chip technology for blood-brain barrier research. Tissue Barriers, 4(1), 2016.
Heidari H, Taylor H., Review Article: Capturing the physiological complexity of the brain’s neuro-vascular unit in vitro, Biomicrofluidics, 12(5), 2018.
Saliba J., Daou A., Damiati S., Saliba J., El-Sabban M., Mhanna R., Development of Microplatforms to Mimic the In Vivo Architecture of CNS and PNS Physiology and Their Diseases, Genes, 9(6), 2018.
Sivandzade F, Cucullo L., In-vitro blood-brain barrier modeling: A review of modern and fast-advancing technologies. J Cereb Blood Flow Metab Off J Int Soc Cereb Blood Flow Metab. 38(10), P. 1667–1681, 2018.
.V. Morgun, V. V. Salmin, A. Y. Uspenskaya, A. E. Teplyashina, A. B. Salmina, Microfluidic technologies in the study and modeling of the blood-brain barrier: Biomedicine • № 4, 2016, P. 22-33.
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