Scholarly record

DEGRADATION POTENTIAL OF BACTERIA AND FUNGI ISOLATED FROM SITES CONTAMINATED WITH PESTICIDES AND SYNTHETIC POLYMERS

PhD. Zuzana Rybkova, Prof. Katerina Malachova, Assoc. Prof. Cenek Novotny

Abstract

The potential for biodegradation of chemically different dyes of six fungi and bacteria isolated from organic pollutants- and synthetic polymer-contaminated sites was determined. Standard enrichment isolation methods using low-nutrient-concentration media were used to obtain bacterial and fungal organisms from environmental samples. The isolates were taxonomically characterized using molecular analysis and Biolog identification system. The isolates included bacterial strains of Pseudomonas putida, Pseudomonas viridilivida, and Enterobacter cloacae, the fungal isolates were Fusarium solani, Trichaptum abietinum, and Trametes suaveolens. The bacterial isolates efficiently degraded Methyl Red, Methylene Blue, and Congo Red dyes. The best decolorization capacity of the fungal strains was detected with Methyl Red, Methylene Blue, Azure B, Disperse Blue 3, and Naphthol Blue Black. These data qualify the strains for the application to the decolorization of synthetic dyes in industrial effluents. The bacterial and fungal isolates obtained in this study may serve as microbial resources for further research aimed at the development of a technology applicable to remediation of colored effluents or biodegradation of other recalcitrant organopollutants.

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