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CULTIVATION OF FUNGI CROPS ON HOLLOW CELLULOSE TUBES AS A MODEL OF THE ROOT SYSTEM OF A PLANT
Abstract
The ability to colonize on plant roots is recognized as one of the most important characteristics of the beneficial fungi. To study these properties, we have developed and created a system for cultivating fungal microorganisms under sterile conditions on the surface of cellulose cylindrical tubes, into which a nutrient solution simulating plant exudate was constantly fed. This system simulates the development of microorganisms on the surface of the root system and allows to study the interaction of microorganisms with each other during their surface growth with a constant supply of exudates to it. Trichoderma viride and Fusarium oxysporum were selected as objects for studying the interaction when they grew on nutrient medium simulating water-soluble exudates of cucumber. The concentration of the model of cucumber exudates in the supplied solution corresponded to 250 mg/l. Growth kinetics studies using this cultivation method have shown that Trichoderma viride is able to grow under such conditions, completely covering the surface of the cellulose tube. The synthesis of enzymes of the lytic series, even at exudate concentrations of about 50 mg/l, was not observed, which indicates the presence of the effect of catabolic repression in this strain, even at such low concentrations of exudates. Studies of the interaction of Trichoderma viride with Fusarium oxysporum, after adding the latter to the surface formed by the mycelium of Trichoderma viride, showed that Fusarium oxysporum cells were not detected after a day of co-cultivation. Thus, the presented model of the method of cultivation of fungal cells made it possible to elucidate the interaction of Trichoderma viride and Fusarium oxysporum during their co-cultivation on media simulating cucumber exudates. The study showed that a physical model could be used to make reasonable predictions about the behaviour of microorganisms in soil?root systems.
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References5
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