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EFFECTIVE GLUCOSE OXIDATION WITH MAGNETICALLY RECOVERABLE BIOCATALYSTS
Abstract
Here, we developed an effective D-glucose oxidation with magnetically recoverable biocatalysts. These biocatalysts were synthesized on magnetite particles obtained by different methods, polyol and co-precipitation. These MPs were coated with a layer of silica modified with amino groups. The GOx covalent attachment has been carried out via the reaction with glutaraldehyde. We established that the method of MPs synthesis affect the enzyme catalytic activity. It should be noted that the magnetically recoverable biocatalyst Fe3O4-polyol/GOx showed high activity - 86% of the activity of the native enzyme.
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