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VITAMIN B12 FERMENTATION PROCESS ON SUNFLOWER PROTEIN ENZYMATIC HYDROLYSATES
Abstract
Bacillus megaterium is a bacterium that has been used for the industrial production of vitamin B12 (cobalamin), the anti-pernicious anaemia factor. Cobalamin is a modified tetrapyrrole with a cobalt ion coordinated within its macrocycle. Previous findings outline a potential way to develop B. megaterium as a cell factory for cobalamin production using cheap raw materials. In this paper, a novel strategy was implemented to investigate the possibility to use sunflower protein isolates and its enzymatic hydrolysates for enhanced B. megaterium fermentation for vitamin B12 production. Compared to the fermentation process with meat peptone, which is traditionally used for the B. megaterium fermentation, it was observed that its substitution with unpurified sunflower protein isolate did not significantly influent the vitamin B12 biosynthesis. Treatment of unpurified sunflower isolate with commercial industrial proteases (Fermgen, Proteinase T, Protex 40E, Protex 51 FP, Protex 7L) showed the increment in ammonia nitrogen concentration in enzymatic hydrolysates. Further application of sunflower protein hydrolysates as a medium for fermentation showed positive effect on B. megaterium cell growth and could be a good candidate for industrial fodder, enriched with vitamin B12.
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https://www3.nuseed.com/eu/global-trends-in-sunflower-production/
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