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THE DESIGN OF MAGNETICALLY SEPARABLE BIOCATALYST ON THE BASIS OF GLUCOSE OXIDASE
Abstract
Here we report immobilization of GOx on magnetic silica: (i) nanoparticles Fe3O4 with silica coating (Fe3O4-SiO2) and (ii) mesoporous silica with incorporated nanoparticles Fe3O4 (SiO2-Fe3O4) functionalized with amino groups using glutaraldehyde as a linker. Magnetic support based biocatalysts demonstrate high catalytic activity in D-glucose oxidation to D-gluconic acid at pH 5-7.5 and temperature of 30-50?C with the best activities of 95% and 94% for SiO2-Fe3O4-GOx and Fe3O4-SiO2-GOx, respectively. A comparison of magnetic and non-magnetic silica shows a significant enhancement of the relative catalytic activity for magnetic supports. Noticeably higher activity of GOx immobilized on magnetic supports is explained by the synergy of the GOx inherent activity and enzyme-like activity of iron oxide nanoparticles. Excellent relative activity of SiO2-Fe3O4-GOx (95% of native GOx) in a tolerant pH and temperature range, as well as high stability in a repeated use (6% relative activity loss after ten catalytic cycles), makes this catalyst promising for practical applications.
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