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METAGENOMIC ANALYSIS OF DIVERSITY OF PROKARYOTES ARTIFICIAL AQUABIOCENOSIS
Abstract
The qualitative and quantitative composition of biomass of microorganisms has been studied in key points of artificial biocenosis: water filter, duckweed and pond snail. Metagenomic studies have shown the presence of Bacteroides and Proteobacteria types in all examined systems, however, specific representatives - Actinobacteria, Cyanobacteria, and Bacilliwere also found, respectively. A microflora peculiarity of water filter was the presence of actinobacteria. In the intestine of the pond snail (Lymnaea stagnalis L.), a significant proportion of gram-positive bacteria belonging to the bacilli, determining symbiotic and associative microflora, was found. In duckweed (Lemna minor L.), representatives of Cyanobacteria were found. In addition, representatives of Bacteroides and Proteobacteria types were found for all three biotopes. Bacteroides prevailed in water filter and Proteobacteria were most characteristic for living objects (Lymnaea stagnalis L., and Lemna minor L.) but differing in the share of its constituent classes for every ecological niche. The data obtained allow us to use marked taxonomic groups of bacteria as identifiers of main zones of artificial biocenosis and to evaluate changes in them in the future by a shift in the qualitative and quantitative composition of microflora representatives.
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