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DEVELO PMENT OF NEW ENVIRONMENTALLY NATURAL INSECTICIDES PRODUCT FROM CONIFEROUS BIOMASS AGAINST INSECT PESTS
Abstract
To protect plants from harmful activity of pests and diseases, more attention should be paid to development and establishment of environmentally friendly regulation actions. Conifer trees produce a wide variety of compounds, such as terpenoids and phenolics. Several studies on coniferous components fungicidal and insecticidal activity are known Since 2010, LU Institute of Biology in cooperation with Latvian Forest Research Institute ?Silava? are working on the development of new environmentally friendly plant protection products from coniferous extracts against pathogenic fungi and pest insects. General aim of the framework of current study is development of new, innovative plant protection products for agriculture, thus facilitating the integration of science and production for sustainable usage of forest resources and development of integrated and biological agriculture. Pine (Pinus sylvestris L.) and spruce (Picea abies (L.) Karst.) processing waste products (needles and bark) of were tested. Different solvents (ethanol, NaOH) were used for extraction. Fungicidal activity of extracts was proven. During 2013 to 2017 several laboratory investigations (bioassays) were carried out to test the effectiveness of selected coniferous extracts against pest insects: Acanthoscelides obtects, Pieris brassicae, Trialeurodes vaporariorum, Myzus persicae and Schizaphis graminum. Different extract concentrations were tested, cumulative mortality were calculated. We observed low impact on Pieris brassicae and Acanthoscelides obtects larvae. Bark ethanol extract cause significant mortality of whiteflies and aphids. We also tested impact of extracts on mycelia growth of entomopathogenous fungi, used in pest control. The inhibition of intensity of mycelium growth, sporulation and spore germination was determined for the entomopathogenic fungi: Beauveria bassiana, Lecanicillium lecani, Paecilomyces fumosoroseus and. Aspergillus niger.
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