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BACTERIAL ABUNDANCE, BIOMASS AND PROPORTION OF LIVING CELLS IN LITTORAL SANDY SEDIMENTS ON THE ROMANIAN BLACK SEA COAST

Dan Răzvan Popoviciu

First published: 2011-06-20https://doi.org/10.5593/sgem2011/s14.101View metrics

Abstract

Bacterial density, biomass, morphological dive rsity and cell integrity were studied in surficial sediment samples taken form eight locations on the Romanian coast. Investigation was done by epifluorescence microscopy, employing SYBR Green I (universal fluorochrome) and propidium iodide (for visualising cells with damaged membranes). Cell density varied between 4.62-9.64 Г— 107 cells/cm3 sediment, showing a relation to sediment grain size. Dry biomass ranged between 3.03-6.39 Вµg/cm 3. Rod shaped bacteria were dominant (58-65%), the rods : cocci ratio also having a correlation to sediment granulometry. A significant proportion of the microorganisms observed lacked cell membrane integrity (i.e. dead cells).

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Title
BACTERIAL ABUNDANCE, BIOMASS AND PROPORTION OF LIVING CELLS IN LITTORAL SANDY SEDIMENTS ON THE ROMANIAN BLACK SEA COAST
Authors
Dan Răzvan Popoviciu
Proceedings
SGEM International Multidisciplinary Scientific GeoConference EXPO Proceedings; SGEM2011 11th International Multidisciplinary Scientific GeoConference
Publisher
Stef92 Technology
Year
2011
Pages
Not available yet
ISSN
1314-2704
ISBN
Not available yet
Language
en
Publication type
Conference Paper
Keywords
bacteriaabundancebiomassmembrane integritysandy sediments
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