Scholarly record
SCREENING OF THE ABILITY OF RHIZOSPHERE BACTERIA TO PRODUCE BIOSURFACTANTS
Abstract
Surfactants produced by microorganisms, known as biosurfactants, have recently found wide application in the industry, from cosmetology to oil processing. Biosurfactants are more tolerant to extreme environmental conditions and less hazardous for the environment as compared with their chemical analogues. Despite on the fact that many biosurfactant producers have been recently isolated and described, the challenge to explore new producers still remains very urgent. In the present study, the isolates obtained from rhizosphere soil were screened towards their abilities to emulsify oil and to decrease interface tension between oil and water. As a source of potential producers, rhizosphere soil of Latuca sativa was used. 12 rapidly growing isolates were cultivated in glycerol nitrate medium at 28? for 72 h. Emulsification activity (E24) and drop collapse tests were used to screen the abilities of the supernatants obtained. The lowest E24 was registered for 7 of 12 isolates (from 0% to 43%), while the highest one was registered for 5 from 12 isolates (50%). All these 5 isolates (12b, 19a, 20b, 23a and 26b) demonstrated high efficiency in drop collapse test as well (maximal scores in qualitative test). Besides, 3 other isolates were characterized as the most efficient in the drop collapse test (2c, 9a and 17a).
Publication Impact Profile
Publication details
References13
Le Guenic S., Chaveriat L., Lequart V., Joly N., Martin P., Renewable Surfactants for Biochemical Applications and Nanotechnology, Journal of Surfactants and Detergents, vol. 22, no. 1, pp. 5�21, 2019. DOI: 10.1002/jsde.12216
Asaka O., Shoda M., Biocontrol of Rhizoctonia solani Damping-Off of Tomato with Bacillus subtilis RB14, Applied and Environmental Microbiology Journal, vol. 62, pp. 4081�4085, 1996. DOI: 10.1128/aem.62.11.4081-4085.1996
Campos-Takaki G. M., Sarubbo L. A., Albuquerque C. D. C., Environmentally friendly biosurfactants produced by yeasts, Advances in Experimental Medicine and Biology, vol. 672, pp. 250�260, 2010. DOI: 10.1007/978-1-4419-5979-9_19
Singh B. P., Cowie A. L., Long-term influence of biochar on native organic carbon mineralisation in a low-carbon clayey soil, Scientific Reports, vol. 4, p. 3687, 2015. DOI: 10.1038/srep03687
Mulligan C. N., Yong R. N., Gibbs B. F., Surfactant-enhanced remediation of contaminated soil: a review, Engineering Geology, vol. 60, pp. 371�380, 2001. DOI: 10.1016/s0013-7952(00)00117-4
Scott M. J., Jones M. N., The biodegradation of surfactants in the environment, Biochimica et Biophysica Acta - Biomembranes, vol. 1508, pp. 235�251, 2000. DOI: 10.1016/s0304-4157(00)00013-7
Tackie-Otoo B. N., Ayoub Mohammed M. A., Yekeen N., Negash B. M., Alternative chemical agents for alkalis, surfactants and polymers for enhanced oil recovery: Research trend and prospects, Journal of Petroleum Science and Engineering, vol. 187, p. 106828, 2020. DOI: 10.1016/j.petrol.2019.106828
Adu S. A., Naughton P. J., Marchant R., Banat I. M., Microbial Biosurfactants in Cosmetic and Personal Skincare Pharmaceutical Formulations, Pharmaceutical Journal, vol. 12, p. 1099, 2020. DOI: 10.3390/pharmaceutics12111099
Gudina E. J., Rangarajan V., Sen R., Rodrigues L. R., Potential therapeutic applications of biosurfactants, Trends in Pharmacological Sciences, vol. 34, pp. 667� 675, 2013. DOI: 10.1016/j.tips.2013.10.002
Bodour A. A., Drees K. P., Maier R. M., Distribution of biosurfactant-producing bacteria in undisturbed and contaminated arid southwestern soils, Applied and Environmental Microbiology Journal, vol. 69, pp. 3280�3287, 2003. DOI: 10.1128/aem.69.6.3280-3287.2003
Jain D. K., Collins-Thompson D. L., Lee H., Trevors J. T., A drop-collapsing test for screening surfactant-producing microorganisms, Journal of Microbiological Methods, vol. 13, pp. 271�279, 1991. DOI: 10.1016/0167-7012(91)90064-w
Youssef N. H., Duncan K. E., Nagle D. P., Savage K. N., Knapp R. M., McInerney M. J., Comparison of methods to detect biosurfactant production by diverse microorganisms, Journal of Microbiological Methods, vol. 56, pp. 339�347, 2004. DOI: 10.1016/j.mimet.2003.11.001
Varjani S., Upasani V. N., Evaluation of rhamnolipid production by a halotolerant novel strain of Pseudomonas aeruginosa, Bioresource Technology, vol. 288, p. 121577, 2019. DOI: 10.1016/j.biortech.2019.121577
View or Download full articleAccess options
SWS access login
Login as SWS Scientific CommitteeLogin as SWS Scientific PartnerLogin as SWS AuthorAuthors and approved SWS contributors will read and export their own linked papers after identity matching by SWS profile, email and SGEM GlobalID.
For librarian assistance: [email protected]
Purchase Instant Access
- Article can be downloaded after successful payment.
- Article may be used according to SWS library access terms.
- Article cannot be redistributed.

