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APPLICATION OF CULTURE SUPERNATANTS CONTAINING SURFACTANTS TO ENHANCE OIL RECOVERY

Liliya Biktasheva, Nikita Ezhkin, Zlata Osmorskaya, Alexander Gordeev, Polina Kuryntseva

First published: 2024-11-15https://doi.org/10.5593/sgem2024/1.1/s06.67View metrics

Abstract

Currently, a big problem is increasing the production of residual oil, for which so-called tertiary recovery methods are used. One common tertiary recovery method is the use of surfactants. The use of biological surfactants instead of synthetic ones can solve the problem of increasing oil production and reducing the toxic impact on the environment. To obtain biosurfactants, two strains of Bacillus mojavensis and Bacillus amyloliquefaciens were used. These strains are characterized by the ability to produce biosurfactants. Biosurfactants produced by these strains belonged to the group of lipopeptides, represented mainly by surfactin molecules. The qPCR method established that in strains B. mojavensis and B. amyloliquefaciens the number of srfAA and ituC genes responsible for the production of surfactin was 2.8x104 and 1.9x103 gene copies ml-1, and iturin for 4.6x102 gene copies ml-1 in strain B amyloliquefaciens. To evaluate the effectiveness of MEOR, culture supernatant containing biosurfactants was tested on sand cores simulating oil reservoir. The surface tension of the culture supernatants of the B. mojavensis and B. amyloliquefaciens strains was 29.79 - 0.039 and 31.18 - 0.065 mN m-1. When culture supernatants were used, additional oil recovery was 11.1 and 11.8% for B. mojavensis and B. amyloliquefaciens, respectively.

Publication Impact Profile

Dimensions ID: pub.1183084665

Publication details

Title
APPLICATION OF CULTURE SUPERNATANTS CONTAINING SURFACTANTS TO ENHANCE OIL RECOVERY
Authors
Liliya Biktasheva, Nikita Ezhkin, Zlata Osmorskaya, Alexander Gordeev, Polina Kuryntseva
Proceedings
24th International Multidisciplinary Scientific GeoConference Proceedings SGEM 2024, Science and Technologies in Geology, Exploration And Mining, Vol 24, Issue 1.1
Publisher
STEF92 Technology
Year
2024
Pages
541-548
SWS Citekey
Biktasheva20246541548
ISSN
1314-2704; 13142704
ISBN
9786197603675
Language
en
Publication type
Conference Paper
Proceedings contents
Open official contents
Keywords
References9
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