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STUDY OF BIOSENSOR RECEPTOR LAYER COMPONENT STABILITY UNDER THE INFLUENCE OF VARIABLE ENVIRONMENT ACIDITY

Aleksandra Kłos-Witkowska

First published: 2018-06-20https://doi.org/10.5593/sgem2018/6.2/s25.084View metrics

Abstract

The paper presents the impact of variable environment acidity and low temperature on the stability of biosensor receptor layer component BSA (Bovine Serum Albumin). With the use of UV/Vis spectrophotometer, absorption spectra in range (250-300nm) was taken to study conformational changes of the protein with time after exposition to different factors. The results showed an increase in absorption, associated with the conformational changes of BSA with time. A significant decrease in the absorption intensity was noted after the addition to pure BSA - brain metabolite NAA. Performed studies showed that, the increase in the concentration of the NAA brain metabolite caused a decrease in the pH of aqueous solutions of BSA, which correlates the environmental changes to more acidic ones. For samples with higher acidity, the changes in absorption over time were gentle, which may confirm the increased stability of the biosensor matrix factor in an acidic environment. Comparing the effect of low temperature, it could be seen that temperature 4В°C also influence on the protein stability and showed tendency to maintain receptor layer component stable. The observed trend of change, under the influence of external factors was more evident with time.

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Publication details

Title
STUDY OF BIOSENSOR RECEPTOR LAYER COMPONENT STABILITY UNDER THE INFLUENCE OF VARIABLE ENVIRONMENT ACIDITY
Authors
Aleksandra Kłos-Witkowska
Proceedings
SGEM International Multidisciplinary Scientific GeoConference EXPO Proceedings; 18th International Multidisciplinary Scientific GeoConference SGEM2018, Nano, Bio and Green - Technologies for a Sustainable Future
Publisher
STEF92 Technology
Year
2018
Pages
637-644
SWS Citekey
Klos-Witkowska201825637644
ISSN
1314-2704
ISBN
978-619-7408-51-5
Language
en
Publication type
Conference Paper
Keywords
biosensorenvironmental aciditystabilityBSA
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