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EFFECT OF PHENOLIC CONSTITUENTS OF PLANT ORIGIN ON PHAGOCYTIC ACTIVITY OF PERIPHERAL BLOOD CELLS IN VITRO
Abstract
Chemical compounds of natural origin have high biological activity, complex effects on the human body, and safety for health. Preparations based on natural plant raw materials affect the body's adaptive capabilities, acting as factors of nonspecific defense and cofactors of the immune system. The aim of the study is to evaluate the effect of phenolic constituents of plant origin on the phagocytic activity of peripheral blood cells in vitro. Materials and methods. In vitro study of the effect of phenolic constituents of plant origin obtained from the extraction of the Norway spruce cones for the absorptive activity of phagocytes, 13 samples of venous blood were taken. Control samples — samples with no phenolic constituents of plant origin added (n=13), test samples — samples with added phenolic constituents of plant origin at a concentration of 2 mg/ml (n=13). A decoction of the Norway spruce cones was prepared following the requirements of the State Pharmacopoeia of the 14th edition. Results. A statistically significant (p=0.004) decrease of up to 60% from the initial level of phagocytic activity of peripheral blood phagocytes was found upon exposure to phenolic constituents of plant origin. The test results in vitro suggest a change in the oxygen-dependent mechanisms of phagocytosis under the influence of phenolic constituents obtained from natural raw materials of plant origin. Findings. The test results suggest a possible change in the transduction of intracellular signals and the functional activity of professional phagocytes associated with the presence of pronounced antioxidant properties in phenolic constituents of plant origin, leading to inhibition of phagocytic activity in peripheral blood.
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