Scholarly record
DETECTION OF TOXIC EFFECT OF NANOSIZED MAGNESIUM OXIDE ON BRAIN TISSUE IN AN EXPERIMENT USING MULTIFRACTAL HISTOLOGY PREPARATIONS- IMAGE ANALYSIS
Abstract
The article reports on detection of toxic effect of nanosized magnesium oxide on brain tissue of laboratory animals that have been given the substances via inhalation and orally. The experiment was performed on mature adult male Wistar rats (weight 250±15 g) and on BALB/C mice (weight 25±2 g). Detection of the nanoscale magnesium oxide toxic effects on brain tissue was conducted using multifractal image analysis of histology preparations with application of the binarization and constructing Voronoi diagrams from graphical data. As a result, it was found that the nanoscale magnesium oxide toxic effect on the brain through different routes of entry is expressed in the orderliness and homogeneity decrease in tissue structure with the formation of neurons focal clusters. At the same time, the smaller the orderliness and homogeneity of the structure and the larger the tissue clustering area relative to the control are, the stronger the toxic effect is. The most toxic effect on brain tissue is observed with repeated inhalation exposure to nanoscale magnesium oxide. During the experiment it was established that the use of multifractal image analysis of histology preparations for pathological changes interpretation makes it possible to ignore interspecies differences of laboratory animals and to concentrate directly on the results of substance toxic effects on brain tissue. The results of image analysis allow us to quantitatively characterize pathological changes in brain tissue that are consistent with a qualitative visual assessment of morphological changes in histological preparations.
Publication Impact Profile
- Captures
- Mendeley - Readers: 1
Publication details
References6
Sawai J, Kojima H, Igarashi H, Hashimoto A, Shoji S, Takehara A, et al. Escherichia coli damage by ceramic powder slurries. J Chem Eng Japan. 1997; 30(6):1034-1039. DOI: 10.1252/jcej.30.1034.
Medina C, Santos-Martinez MJ, Radomski A, Corrigan OI, Radomski MW. Nanoparticles: pharmacological and toxicological significance. Br J Pharmacol. 2007; 150(5):552-558. DOI: 10.1038/sj.bjp.0707130 pmid: 17245366.
Bertinetti L, Drouet C, Combes C, Rey C, Tampieri A, Coluccia S, et al. Surface characteristics of nanocrystalline apatites: effect of mg surface enrichment on morphology, surface hydration species, and cationic environments. Langmuir. Iran J Biotech. 2019; 17(1):e1543 2009; 25(10):5647-5654. DOI: 10.1021/la804230j pmid: 19281274.
Kakar S, Batts KP, Poterucha JJ, Burgart LJ. Histologic changes mimicking biliary disease in liver biopsies with venous outflow impairment. Mod Pathol. 2004;17(7):874-878. DOI: 10.1038/modpathol.3800073 pmid: 15098006.
Fortune S.J. A sweepline algorithm for Voronoi diagrams // J. Algorithmica. 1987. N 2. P. 153–174..
Mangalampalli B, Dumala N, Perumalla Venkata R, Grover P. Genotoxicity, biochemical, and biodistribution studies of magnesium oxide nano and microparticles in albino wistar rats after 28-day repeated oral exposure. Environ Toxicol. 2018;33.
View or Download full articleAccess options
SWS access login
Login as SWS Scientific CommitteeLogin as SWS Scientific PartnerLogin as SWS AuthorAuthors and approved SWS contributors will read and export their own linked papers after identity matching by SWS profile, email and SGEM GlobalID.
For librarian assistance: [email protected]
Purchase Instant Access
- Article can be downloaded after successful payment.
- Article may be used according to SWS library access terms.
- Article cannot be redistributed.