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NEW POTENTIALLY TOXIC PSEUDO-NITZSHIA SPECIES (BACILLARIOPHYCEAE) IDENTIFIED BY MOLECULAR APPROACH IN THE BLACK SEA (VARNA BAY)
Abstract
The potentially toxic genus Pseudo-nitzschia H.Peragallo, 1900 is common for the phytoplankton community in the Bulgarian part of the Black Sea often proliferating to bloom densities. The highly similar morphological features hamper the precise species discrimination by light microscopy and an accurate identification requires the application of more sophisticated methods. In this study we used genus specific large subunits ribosomal DNA (LSU rDNA) primer pair in order to elucidate the species diversity within Pseudo-nitzschia in Varna Bay. Three Pseudo-nitzschia species (four distinct ribotypes) were found in the constructed from environmental samples clone libraries. Molecular data were compared with light microscopy counts. This is the first detection of P. linea Lundholm, Hasle and G.A.Fryxell, 2002 in the Black Sea and first confirmation of presence of P. calliantha Lundholm, Moestrup & Hasle, 2003 and P. pungens var. aveirensis Lundholm, Churro, Carreira & Calado, 2009 in Bulgarian waters. The results from this preliminary study are in support of the potential of molecular analysis as a powerful approach for clarifying Pseudo-nitzschia taxonomic diversity in Bulgarian Black Sea waters.
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References15
Lelong A., Hegaret H., Soudant, P., Bates S., Pseudo-nitzschia (Bacillariophyceae) species, domoic acid and amnesic shellfish poisoning: revisiting previous paradigms. Phycologia, vol. 51/issue 2, pp 168-216, 2012.
Lundholm N. (Ed)., Bacillariophyceae, in IOC-UNESCO Taxonomic Reference List of Harmful Micro Algae. Available online at http://www.marinespecies.org/hab. Accessed on 2017-04-11.
Moncheva S., Krastev A., Some aspects of phytoplankton long-term alterations off Bulgarian Black Sea shelf. Sensitivity to Change: Black Sea, Baltic Sea and North Sea. Springer Netherlands, pp 79-93, 1997.
Besiktepe S., Ryabushko L., Ediger D., Yilmaz D., Zenginer A., Ryabushko V., Lee R., Domoic acid production by Pseudo-nitzschia calliantha Lundholm, Moestrup et Hasle (Bacillariophyta) isolated from the Black Sea. Harmful Algae, vol. 7, pp 438-442, 2008.
Peneva V., Gogov Y., Kalinova G., Slavova, A., Application of HPLC method for determination of ASP toxins in bivalve mulluscs. The Jubilee Scientific Session — Conference 110 years National Diagnostic Science-and-Research Veterinary Medical Institute Sofia, Bulgaria, 08 - 09.11.2011, pp 210-213.
McDonald S.M., Sarno D., Zingone A., Identifying Pseudo-nitzschia species in natural samples using genus-specific PCR primers and clone libraries. Harmful Algae, vol. 6, pp 849–860, 2007.
Moncheva S., Parr B., Manual for phytoplankton sampling and analysis in the Black Sea, Phytoplankton Manual, UP-GRADE Black Sea Scene Project, FP7 No: 226592 Black sea Commission, 2010.
Tamura K., Stecher G., Peterson D., Filipski A., Kumar S., MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, vol. 30, pp 2725-2729, 2013.
Madden T., The BLAST Sequence Analysis Tool. In: McEntyre J, Ostell J, editors. The NCBI Handbook [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2002. Chapter 16. Available from: http://www.ncbi.nlm.nih.gov/books/NBK21097/
Romari K., Vaulot D., Composition and temporal variability of picoeukaryote communities at a coastal site of the EnglishChannel from 18S rDNA sequences. Limnol. Oceanogr., vol. 49, pp 784–798, 2004.
Librado P., Rozas J. DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, vol 25, pp 1451-1452, 2009.
Baytut O., Moestrup O., Lundholm N., Gonulol, A. Contributions to the diatom flora of the Black Sea from ultrastructural and molecular studies: new records of Skeletonema marinoi, Pseudo-nitzschia pungens var. aveirensis and Chaetoceros tenuissimus for the Marine Flora of Turkey. Nova Hedwigia, vol. 96/issue 3-4, pp 427- 444, 2013.
Ruggiero M.V., Sarno D., Barra L., Kooistra W.H., Montresor M., Zingone A., Diversity and temporal pattern of Pseudo-nitzschia species (Bacillariophyceae) through the molecular lens. Harmful Algae, vol. 42, pp 15-24, 2015.
Tas S., Lundholm N., Temporal and spatial variability of the potentially toxic Pseudo-nitzschia spp. in a eutrophic estuary (Sea of Marmara). Journal of the Marine Biological Association of the United Kingdom, pp 1-12, 2016.
Terenko L., Terenko G.. Dominant Pseudo-nitzschia (Bacillariophyta) species in the Black Sea (Ukraine) – Bot. Lith., vol. 18/issue 1, pp 27–34, 2012.
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