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ESTIMATION OF EFFICIENCY OF USE OF VEGETABLE OILS FOR RE-CEPTION OF BIOFUEL BY A METHOD OF ENZYMATIC CATALYSIS
Abstract
Biodiesel is a relatively new type of environmentally friendly fuel, which is made from renewable resources. Biodiesel is a mixture of methyl or ethyl esters of fatty acids. It is obtained by re-esterification of vegetable oils or others fat-containing substrate, including animal origin. Iodine and acid numbers of vegetable oils, such as rapeseed, olive, linseed, nut and sunflower, are determined. In addition, conditions for obtaining biodiesel by en-zymatic catalysis using pancreatic lipase as an enzyme and rapeseed oil as a substrate for pancreatic lipase were studied. Optimal concentrations of pancreatic lipase and optimal conditions for carrying out the process of transesterification of vegetable oils, in particular rapeseed oil, for obtaining biodiesel fuel were selected. It is established that the maximum degree of hydrolysis by the pancreatic lipase is achieved when using rapeseed, olive and linseed oil. For rapeseed oil the degree of hydrolysis was 57%, for olive oil - 51%, for linseed oil - 48%. A one-stage and two-stage transesterification processes are compared. It has been established that a one-step process should be performed at a concentration of 6% pancreatic lipase, the duration of the process being 8 h with a degree of hydrolysis of 81%.
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