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APPLICATION OF NOVEL SYNTHESIZED LUMINOPHORE AZP5 FOR EFFICIENT STAINING OF TREMATODA: FASCIOLIDAE PARASITES
Abstract
Methods of parasite structures visualization using fluorescent probes are widely used in nowadays. The technological utilization of benzanthrone dyes is growing, but in biology studies they are still rarely used. Our studies show the advisability/expediency of using these dyes in investigation of helminthes structures. The aim of present study was to evaluate the novel synthesized luminophore AZP5 for efficient staining of trematodes for confocal laser scanning microscopy. All helminthes were previously preserved in 96% ethanol, stained in AZP5, washed with 96% ethanol and then with ethanol-xylene, and 100% xylene was added. All specimens were mounted with Canada balsam and were visualized using the confocal laser scanning fluorescence microscopy. Surface of fluke, morphology of musculature, oral and ventral suckers, digestive tract, excretory and reproductive systems were demonstrated in pictures in good quality. The developed staining protocol can be used for samples collected in field studies. Obtained results of the present research may be importantl to design new better luminophores and to expand opportunities of confocal laser scanning microscopy.
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Rao J., Dragulescu-Andrasi A., Yao H., Fluorescence imaging in vivo: recent advances. Current Opinion in Biotechnology, vol. 18, pp. 17–25, 2007,
Terai T., Nagano T., Fluorescent probes for bioimaging applications. Current Opinion in Chemical Biology, vol. 12, pp. 515–521, 2008.
Fei X., Y. Gu. Progress in modifications and applications of fluorescent dye probe. Progress in Natural Science, vol. 19, pp. 1–7, 2009.
Plášek J., Hošková B., Solvatochromic Effect in the Optical Spectra of Calcofluor and its Relation to Fluorescent Staining of Yeast Cell Walls. Journal of Fluorescence, vol. 20, issue 1, pp. 343-352, 2010.
Hageage G. J, Harrington B. J. Use of calcofluor white in clinical mycology. Laboratory Medicine, vol. 15, pp. 109-112, 1984.
Harrington B. J, Hageage G. J., Calcofluor white: a review of its uses and applications in clinical mycology and parasitology. Laboratory Medicine, vol. 34, pp. 361–367, 2003.
Wysocki L.M., Lavis L. D., Advances in the chemistry of small molecule fluorescent probes. Current Opinion in Chemical Biology, vol. 15, pp. 752–759, 2011.
Grabowski Z. R., Rotkiewicz K., Structural Changes Accompanying Intramolecular Electron Transfer Focus on Twisted Intramolecular Charge-Transfer States and Structures, Chem. Rev. vol.103 pp. 3899–4032, 2003.
Shivraj, Siddlingeshwar B., Kirilova E. M., Belyakov S. V., Divakar D. D., Alkheraif A. A., Photophysical Properties of Benzanthrone Derivatives: Effect of Substituent, Solvent Polarity and Hydrogen Bonding. Photochemical & Photobiological Sciences, vol. 17, pp. 453-464, 2018.
Valeur B., Molecular Fluorescence: Principles and Applications, Wiley-VCH, Weinheim, pp. 399, 2001.
Kapusta P., Machalicky O., Hrdina R., Nepras M., Zimmt M. B., Fidler V., Photophysics of 3-Substituted Benzanthrones: Substituent and Solvent Control of Intersystem Crossing, J. Phys. Chem., vol. 107, 9740-9746, 2003.
Slavik J., Fluorescent probes in cell biology, CRC Press, Boca Raton; Ann Arbor; London, Tokyo, pp. 295, 1994.
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