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RESEARCH ON THE AEROBIC BACTERIAL FLORA ISOLATED FROM CATTLE WITH CLINICAL ENDOMETRITIS
Abstract
In cattle, uterine infections are one of the major causes of reproductive disorders. These bacterial infections are frequently treated with antibiotics, but their effectiveness must be evaluated, due to the continuous emergence of bacterial strains with multiple drug resistances. The research was conducted on several 190 samples from cows with a presumptive diagnosis of endometritis. The exploitation technology practiced at this farm is the free stall type. Samples were collected using sterile swabs and transported under appropriate conditions to the laboratory. The definitive identification of suspected etiological agents was made with the help of diagnostic kits: API 20 E - for the identification of pathogens from the Enterobacteriaceae family, API Staph - for the identification of staphylococci (API - Analytical Profile Index). Out of the total 190 processed samples, more than half of them were gram-negative bacteria, 105 strains (55.26%) and 85 strains (44.74%) were gram-positive bacteria. From the 190 samples collected from cows with endometritis, 95 strains of Escherichia coli (50%), 20 strains of Staphylococcus spp. (10.52%), 37 strains of Klebsiella spp. (19.47%) were isolated, 19 strains of Streptococcus spp. (10%) 19 strains of Pseudomonas spp. (10%). The isolated strains had similar behavior, being sensitive to enrofloxacin, florfenicol, gentamicin, and lincospectin at 94.44%. Most of the gram-positive bacterial strains showed sensitivity to ceftiofur, cefoxitin, and cefotaxime. Among gram-negative bacterial strains, resistance to amoxicillin / clavulanic acid was found to be 100%, penicillin at 77%, and ampicillin at 72%. Among the gram-positive bacterial strains, 78.57% penicillin and 71.43% amoxicillin/clavulanic acid resistance were found.
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