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THE STUDY OF BIOSTIMULATING EFFECT OF POROUS CERAMICS FUNCTIONALIZED WITH POLYOXOMETALATES

Cristina Roșu

Abstract

For the analysis of polyoxometalate functionality (POM), a bioassay was developed in order to determine the effect of the functionalized porous ceramic granule (with POM) on the germination of tomato plantlets (Lycopersicon esculentum) and cowpea (Vigna unguiculata). After 3 days of incubation under dark conditions at room temperature in the case of cowpea seeds and 6 days in the case of tomato seeds, the germination process was calculated and the radicle and hypocotyl growths of the obtained plantlets were biometrically assessed in each of the treatment variants respectively, in the control sample (distilled water). When the percentage of germination was assessed, only the seeds with a germ larger than 2 mm in length were considered germinated. Analyzing the influence of the suspensions added to the germination substrate, it was found that, in case of the cowpea, after the seed germination time (3 days), the supernatant treatments obtained by suspending 5g of porous ceramic granules functionalized with POM in 100 ml of distilled water for 2 hours generally stimulated germination percentage, this being 100% in most experimental variants. Only at POM2, the maximum concentration, the germination value was below 100%, but it was above that of the untreated control sample (AA). In case of tomatoes, the percentage of germinated plantlets was between 96-100% after 6 days of incubation. And in the case of cowpea, the supernatant treatments obtained by suspending 5 g of porous ceramic granules functionalized with POM in 100 ml of distilled water for 2 hours generally stimulated the germination percentage. Only treatment variants in which 10% supernatant (2 ml to 20 ml medium) from POM2 supernatant was added to the substrate, it showed a germination of less than 100%, but also an increase compared to the untreated control sample (AA) and even towards the control sample represented by the non-functionalized ceramic supernatant. Regarding the size of the hypocotyls and radicle, those were biometrically assessed by measuring at the time of full germination, after three days in the case of the cowpea and 6 days in the case of tomatoes. These morphological analyzes demonstrate a significant biostimulation effect of nutrients released into the supernatant obtained by suspending 5g porous ceramic granules functionalized in 100 ml distilled water for 2 hours. The proposed bioassay are quick, easy to achieve, and represent the optimal way of ensuring the quality required for the reproducibility of the polyoxometalate functionality which were included in the ceramic granules. For both types of seed used, the functionalized ceramics had a similar behavior.

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DOI resolver: doi.org/10.5593/sgem2018/6.2/s25.098

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