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USE BIOCHEMICAL PARAMETERS FOR IDENTIFICATION OF NEW GRAM-POSITIVE ISOLATES AND THEIR VARIABILITY
Abstract
The diversity of sites with a high proportion of metals is characterized by the occurrence of specifically adapted microorganisms. Their identification and subsequent accurate genotypic and phenotypic characteristics are a prerequisite for further practical applications, especially in bioremediation processes. The article deals with evaluation of biochemical features for fenotypic identification of bacterial microorganisms. 30 new isolates of gram-positive microorganisms of genus Arthrobacter, Bacillus, Brevibacterium and Paenibacillus from the sludge lagoon after extraction and treatment of polymetallic ores showed variable biochemical features (arbutin, ?-cyclodextrin and ?-cyclodextrin, m-inositol, salicin, uridine) that make identification of these isolates more difficult due to their phenotypic characteristics using a BiologTM MicroStation system. The results of the study are related to the unstable genomic regions, especially with the verification of the presence of metal resistance genes in operons of isolated bacterial microorganisms.
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