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COMPARISON OF METHODS OF DNA EXTRACTION FROM CRUDE OIL FOR THE FOLLOWING EXPLORING OF ITS BIODIVERSITY
Abstract
The anthropogenic influence on petroleum reservoirs during oil extraction leads to the emergence of new electron acceptors and donors and exogenous microorganisms. Microbial biodegradation of hydrocarbons in petroleum reservoirs leads to a change of its composition, resulting in a decrease in its cost. The identification of microorganisms capable of in-situ degradation of hydrocarbons under reservoir conditions is critical to understand the role of microbes in hydrocarbon conversion. To analyze microbial biodegradation, it is necessary to have information about the composition and structure of microbial communities in petroleum reservoirs. The most complete analysis of the microbial community, the metagenome analysis, can be carried out using the highthroughput sequencing methods. Before sequencing, isolation of DNA should be carried out, and this is a separate task for research due to its difficulty. There are two approaches for DNA extraction - the first is the analysis of the metagenome of the original oils, which allows to deeply explore their biodiversity. The second approach is to conduct pre-cultivation and enrichment of oil microorganisms. The purpose of this study was to compare total DNA extraction methods from oil samples for subsequent analysis of the metagenomes of flooded producing wells. Eighteen crude oil samples were taken from different wells of the Romashkinskoye oil field (Russia) under anaerobic conditions. The direct extraction was successful only for two of them, once using isooctane and once using Winogradsky-s salt solution as eluents. For the other samples, anaerobic enrichment was implemented, is allowed to obtain DNA from 6 more samples. For the last 10 samples, aerobic enrichment was demonstrated to be successful. However, this method of enrichment purely corresponds to the original conditions of the oil well. Further optimization of the direct DNA extraction from crude oil are required.
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Korenblum E., Souza D.B., Penna M., Seldin L. Molecular Analysis of the Bacterial Communities in Crude Oil Samples from Two Brazilian Offshore Petroleum Platforms // Int. J. Microbiol., vol. 2012, �� 1�8, 2012. DOI: 10.1155/2012/156537
Connan J. BIODEGRADATION OF CRUDE OILS IN RESERVOIRS. // Advances in Petroleum Geochemistry, 1984. DOI: 10.1016/b978-0-12-032001-1.50011-0
Magot M., Ollivier B., Patel B.K.C. Microbiology of petroleum reservoirs // Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 2000.
Wang L.-Y., Ke W.-J., Sun X.-B., Liu J.-F., Gu J.-D., Mu B.-Z. Comparison of bacterial community in aqueous and oil phases of water-flooded petroleum reservoirs using pyrosequencing and clone library approaches // Appl. Microbiol. Biotechnol., vol. 98(9), pp 4209�4221, 2014. DOI: 10.1007/s00253-013-5472-y
da Cruz G.F., de Vasconcellos S.P., Angolini C.F.F., Dellagnezze B.M., Garcia I.N.S., de Oliveira V.M., dos Santos Neto E. V., Marsaioli A.J. Could petroleum biodegradation be a joint achievement of aerobic and anaerobic microrganisms in deep sea reservoirs? // AMB Express, vol. 1(1), pp 47, 2011. DOI: 10.1186/2191-0855-1-47
Berdugo-Clavijo C., Gieg L.M. Conversion of crude oil to methane by a microbial consortium enriched from oil reservoir production waters // Front. Microbiol., vol. 5, pp 10, 2014. DOI: 10.3389/fmicb.2014.00197
Pannekens M., Kroll L., Muller H., Mbow F.T., Meckenstock R.U. Oil reservoirs, an exceptional habitat for microorganisms // N. Biotechnol., vol. 49, pp 1�9, 2019. DOI: 10.1016/j.nbt.2018.11.006
De Oliveira V.M., Sette L.D., Simioni K.C.M., Neto E.V.D.S. Bacterial diversity characterization in petroleum samples from Brazilian reservoirs // Brazilian J. Microbiol., 2008. DOI: 10.1590/s1517-83822008000300007
Sierra-Garcia I.N., Belgini D.R.B., Torres-Ballesteros A., Paez-Espino D., Capilla R., Santos Neto E. V., Gray N., de Oliveira V.M. In depth metagenomic analysis in contrasting oil wells reveals syntrophic bacterial and archaeal associations for oil biodegradation in petroleum reservoirs. // Sci. Total Environ., vol. 715. pp 136646, 2020. DOI: 10.1016/j.scitotenv.2020.136646
Dellagnezze B.M., Vasconcellos S.P., Angelim A.L., Melo V.M.M., Santisi S., Cappello S., Oliveira V.M. Bioaugmentation strategy employing a microbial consortium immobilized in chitosan beads for oil degradation in mesocosm scale // Mar. Pollut. Bull., vol. 107(1), pp 107� 117, 2016. DOI: 10.1016/j.marpolbul.2016.04.011
Liang B., Zhang K., Wang L.-Y., Liu J.-F., Yang S.-Z., Gu J.-D., Mu B.-Z. Different Diversity and Distribution of Archaeal Community in the Aqueous and Oil Phases of Production Fluid From High-Temperature Petroleum Reservoirs // Front. Microbiol., vol. 9(841), pp 12, 2018. DOI: 10.3389/fmicb.2018.00841
Nazina T.N., Shestakova N.M., Pavlova N.K., Tatarkin Y.V., Ivoilov V.S., Khisametdinov M.R., Sokolova D.S., Babich T.L., Tourova T.P., Poltaraus A.B., Belyaev S.S., Ivanov M.V. Functional and phylogenetic microbial diversity in formation waters of a low-temperature carbonate petroleum reservoir // Int. Biodeterior. Biodegradation, vol. 81, pp 71�81, 2013. DOI: 10.1016/j.ibiod.2012.07.008
Semenova E.M., Ershov A.P., Sokolova D.S., Tourova T.P., Nazina T.N. Diversity and Biotechnological Potential of Nitrate-Reducing Bacteria from Heavy-Oil Reservoirs (Russia) // Microbiology, vol. 89(6), pp 13, 2020. DOI: 10.1134/s0026261720060168
von der Weid I., Korenblum E., Jurelevicius D., Rosado A.S., Dino R., Sebastian G.V., Seldin L. Molecular diversity of bacterial communities from subseafloor rock samples in a deep-water production basin in Brazil // J. Microbiol. Biotechnol., vol. 18(1), pp 5�14, 2008.
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