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NITROGEN COMPOUNDS PATHWAY DURING BIOTRANSFORMATION IN WASTEWATER TREATMENT SYSTEM OF PULP AND PAPER INDUSTRY
Abstract
The main factor of effective biological waste water treatment and pollutants biotransformation is correlation between different nitrogen forms in the media. The waste water specimens were obtained from the pulp and paper mill of the Russian North involving BAS-technology. Concentration of ammonium ions in wastewater was carried out on a spectrophotometer by Sedji-Solorzano method using phenol-hypochlorite reaction. Nitrite ions were determined colorimetrically by diazotization with sulfanilamide and subsequent coupling with N-(1-naphthyl) ethylenediamine to form an intensely-colored azo dye (Greiss reaction). The sodium salicylate method was used for nitrate determination. It was found that organic nitrogen consumption followed by ammonium nitrogen formation occurred during wastewater treatment. The amount of ammonium was insufficient for development of active sludge biomass that required additional introduction of aqua ammonia. The increasing of ammonium nitrogen occurred during ammonification as a result of organic nitrogen transformation. The decreasing of ammonium ion concentration and growth of active sludge biomass in aerobic condition occurred in the bioreactor. The nitrite concentration during biological treatment was low that indicates stable nitrification providing quick transformation unstable nitrite ions to nitrate ions. As a result of organic substances assimilation by active sludge microorganisms the concentration of total nitrogen in dissipating output water was decreased almost twofold.
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