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THE EXTRACTION OF DNA FROM COLONIES OF PECTINATELLA MAGNIFICA (LEIDY 1851)

Vendula Moravcova, Lenka Havlickova, Josef Rajchard

Abstract

Pectinatella magnifica is freshwater organism, which round-shaped colonies are most often found attached to the different submerged parts of coastal plants and can reach the weight even several kilograms. Usually it can be found in oligotrophic to mesotrophic waters, during the season when water temperature reaches 20пїЅC and more [4]. Although Pectinatella magnifica originally comes from North America, it has recently been known as an invasive species in Europe and part of Asia. The first recorded occurrence in Europe was in 1983 near Hamburg [2] in the Czech Republic it was recorded for the first time in rivers Vltava and Labe in the first half of the 20th century [9]. The most common way of its propagation is through internal buds, called statoblasts, which are fitted with hooks. These anchor-shaped hooks make statoblasts able to catch to any surface, and allow easy spread over long distances and different terrain. The aim of this study was to compare different methods of isolation of DNA from colonies of Pectinatella magnifica. Three main different techniques of DNA extraction from zooids and statoblasts were tested, namely two kit-based methods: DNeasy Plant Mini isolation kit (QIAGEN), Nucleo Spin isolation kit (Marcherey-Nagel) and one isolation method based on cetyltrimethylammonium bromide (CTAB-PVP). There were also tested differences between statoblast and zooid DNA and between natural stored and ethanol fixed samples. Material for analysis was collected at the Protected Landscape Area (PLA) and a Biosphere Reserve (BR) of T?ebo?sko.

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DOI resolver: doi.org/10.5593/sgem2017/52/s20.126

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