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The immobilization of glucose oxidase (GOx, enzyme of oxidoreductases class) was carried out on non-magnetic (SiO2, Al2O3) and magnetic mesoporous oxides (Fe3O4-SiO2, Fe3O4-Al2O3). Magnetic supports were synthesized by in-situ crystallization of magnetite nanoparticles in mesopores. The surface of the support was preliminarily modified with amino groups and the glutaraldehyde was used as a linker for covalent binding of the enzyme. The supports and biocatalysts were fully characterized using a combination of physi...

Porous supports including oxides and polymers have been employed for enzyme immobilization for many years. In recent times, the research focus has been placed on hierarchical structures, dependencies on pore sizes, and the use of magnetic porous oxides for magnetic recovery. The presence of pores or cavities where enzymes could locate to create an optimal degree of crowding achieved in cells is not a prerequisite, but it could significantly enhance the catalytic activity of biocatalysts. For the last five years, t...