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VALIDATION OF REFERENCE GENES FOR RT-QPCR IN PORCINE OOCYTES CULTURED IN ANTIOXIDANT-ENRICHED MEDIA
(STEF92 Technology, 2024-12-15, Oana Maria Boldura, Jelena Savici, C. Mircu, Cornel Baltă, Simona Marc)
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Reference genes are essential for normalising data in RT-qPCR experiments and are critical in reducing technical variability. This study aimed to identify and validate stable reference genes for accurate gene expression analysis in porcine oocytes matured in culture media supplemented with antioxidants. Five commonly used reference genes were assessed: PPIA, RPL4, GAPDH, YWHAZ, and TBP, [2, 3] in oocytes cultured under oxidative stress conditions induced by Vitamin C. The experiment involved four experimental grou...
Ecology and Environmental Protection2024