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Estradiol, also designed as 17- ? -estradiol, belongs to the pharmaceutical class of steroid estrogens and was included in the -Watch List- since 2013 the Directive 2013/39/EU due to its potential risk to human health and environment. The low removal efficiency of estrogens by the conventional wastewater treatment plants, becomes a major source of their release into different aquatic matrices. This work presents the optimization of an analytical methodology based on solid phase extraction and high-performance liqu...