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Water Framework Directive 2000/60 / EC is considered a progressive and innovative legislative measure which should lead to improved of surface water in Europe. One important factor contributing to the impairment of surface water quality is the use of chemical and organic fertilizers, which through runoff or leaching in water table can cause the phenomenon of pollution of surface waters and groundwater. In a river basin, the factors that determine the nutrient loads are: industry, urban sewage, atmospheric depositi...