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Conventional wastewater treatment plants (CWWTPs) play a key role in reducing the environmental impact of urban wastewater. But nowadays, this role goes even further with climate change, water scarcity, and the current energy crisis. After the thickening of the secondary sludge, a wastewater flow is generated, which is normally recirculated to the plant head for further treatment. This work aims to use secondary thickener wastewater (STW) as a culture medium for Chlorella vulgaris, Neochloris oleoabundans and Scen...