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Abstract. The possibility of obtaining biofuel and producing electricity in microbial fuel cells is shown when using the industrial cellulose-destroying Clostridium acetobutylicum strain VKPM-4786 as a bioagent. Substrates for microorganisms in MFC were cellulose, glucose, birch sawdust, starch, sludge-lignin, substandard potatoes, peptone. Calculation of the power of the MFCs under investigation (for 2 days of work) showed that the most effective among them was MFC based on a liquid nutrient medium with the addit...