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PRESERVATION OF FOREST GENETIC RESOURCES OF POPULUS NIGRA L. THROW IN VITRO CULTURE

Lucia Ioniță

Abstract

The possibility of regeneration of plants from somatic and genetic cells open the way of in vitro storage of the genotypes. The utilisation of such a method has a lot of advantages as: relatively reduced space for preservation of a large number of multiplicated clonal plants, the plants are preserved free of pathogens and viruses, in special storage conditions, the plants don?t need special treatments during preservation, the material has an adequate form for establishing nuclear stocks which make possible rapid propagation of a very large number of plants. The in vitro of black poplar cultures were initiated from dormant buds and nodal segments which were harvested from selected forest genetic resources. We tested the influence of different factors on the initiation in vitro cultures: the type of initial explants, the sterilisation method and the culture medium. The culture medium we utilised was MS medium supplemented with different hormonal balances, for establishing the optimum composition for initiation of in vitro cultures for this forest species. The percentage of survival was sometimes up to 100%. These cultures were transferred on multiplication medium, the multiplication coefficients being relatively high and varying with the culture medium used. The multiplicated plants were rooted, this step being essential for the success of the conservation of genetic resources throw in vitro cultures. We studied, also, the influence of the culture medium on rooting of the in vitro plants and we established the optimal condition for plant regeneration from in vitro cultures.

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DOI resolver: doi.org/10.5593/sgem2018/6.2/s25.061

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