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COMPARISON OF TWO METHODS OF PURIFICATION OF LIPOPEPTIDES PRODUCED BY BACILLUS SUBTILIS HZMJW 1-10 ISOLATED FROM OIL-CONTAMINATED SOIL
Abstract
Biosurfactants produced by soil microorganisms are now widely used in various industries and are able to compete with synthetic detergents. However, in contrast with chemical surfactants, biological ones have disadvantage: it is difficult to obtain the product of high purity since the microbes usually produce a complex mixture of compounds, besides the cultural medium itself contains a lot of various components. Thus, the conventional scheme of lipopeptide isolation involves acid precipitation, extraction with the solvent, filtration and drying. However, it is reported that often the biosurfactants obtained still contain a lot of impurities, and new methods are required to obtain the compounds of higher quality. This work presents the application of a new method for purifying of lipopeptides produced by Bacillus subtilis strain HZMJW 1-10 to obtain highly pure product. It was compared with the conventional method, which yielded up to 100 mg l-1, while the yield of the new product was only 9 mg l-1. However, the product was completely colorless, did not contain foreign impurity phases, that can influence biological and physical properties. Emulsifying activity (E24) with crude oil at 100 ppm was estimated to be 60% and 85% for the conventional and new products, respectively. FTIR spectrum of the new product contained less peaks in the non-specific regions as compared with that of the conventional one.
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