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ISOLATION OF PANCREATIC LIPASE FROM THE PANCREAS OF CATTLE DURING ITS COMPLEX PROCESSING
Abstract
The pancreas of cattle is one of the most important by-products produced in the meat processing industry. Meat processing plants of medium capacity per year produce at least 40 tons of pancreas of cattle. The pancreas is a valuable raw material for the biotechnology, because it has high contents of hydrolytic enzymes. Among them, the most important enzymes are ribonuclease, proteases, amylase and lipase. The most perspective is the implementation of low-waste technology, involving the isolation of the maximum number of enzymes in a single technological process. The purpose of this work is to select the conditions for the isolation of pancreatic lipase from the pancreas, which is widely used in the production of detergent powders, the food industry, and the production of biodiesel. During the course of the investigations, a sequence of pancreas treatment was established. According to this sequence, during the first stage, amylase is isolated by treating the pancrease with aqueous-salt solutions. In the second stage, lipase is isolated by treating the solid pancreas residue with organic solvents. The conditions for isolating lipase from the pancreas, namely the type of organic extractant, the temperature of the process, the concentrating of the lipase solution by ultrafiltration, and precipitation are selected. As a result of the investigations, a sample of lipase with an enzymatic activity of at least 2000 U / g was obtained, which was successfully tested for the hydrolysis of fat-containing wastes of the meat processing industry.
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References8
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