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Surfactants as detergents and emulsifying agents are used in various fields of industry and agriculture. These amphiphilic compounds contain hydrophobic and hydrophilic fragments and are usually produced by large-scale chemical synthesis processes. However, recently there is a request to develop biotechnological methods to obtain surfactants, since biosurfactants have many advantages above their chemical analogues - higher tolerance to extreme temperatures, pH and salinity, higher stability and lower or zero negat...

Biosurfactants produced by soil microorganisms are now widely used in various industries and are able to compete with synthetic detergents. However, in contrast with chemical surfactants, biological ones have disadvantage: it is difficult to obtain the product of high purity since the microbes usually produce a complex mixture of compounds, besides the cultural medium itself contains a lot of various components. Thus, the conventional scheme of lipopeptide isolation involves acid precipitation, extraction with the...