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Speciation of heavy metals in bottom sediments defines their ecotoxicity and mobility and therefore needs to be studied thoroughly. Usually single and sequential extraction techniques are used to determine water-soluble, exchangeable, specifically sorbed and carbonates, bound to organic matter, bound to Fe and Mn hydroxides and residual forms. Bottom sediments of the Vyshnevolotsky reservoir (the Upper Volga, the Central region of Russia) were chosen for the research. Lithological and mineral composition of sample...