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ANALYSIS OF CRISPR CASSETTE ELEMENTS IN NATIVE ISOLATES OF SINORHIZOBIUM MELILOTI ISOLATED IN THE CENTRAL ASIAN ORIGIN OF PLANT DIVERSITY
(STEF92 Technology, 2023-10-01, Elizaveta Pernak, Maria E. Vladimirova, Viktoria Muntyan, Alexey М. Afonin, Marina L. Roumiantseva)
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CRISPR-Cas adaptive immunity systems of prokaryotes have been identified in 42% of bacteria and 85% of archaea [1] and are actively used in genetic engineering. However, in nitrogen-fixing nodule bacteria required for agriculture, these systems are very poorly characterized. Therefore, in this study, we evaluated the diversity of CRISPR-Cas systems in the genomes of 39 isolates of alfalfa nodule bacteria Sinorhizobium meliloti recovered from soil samples from Kazakhstan and Turkmenistan, which belong to the Centra...
Advances in Biotechnology2023