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Polycyclic aromatic dyes, are widely used as fluorescent labels, and of super-resolution microscopy. Dyes exhibit high photo stability, broad emission spectra making them suitable for advanced bioimaging. That is especially important in parasitology, where fluorescent labelling enables detailed investigation of pathogen morphology, and dynamic biological processes in living systems. In previous studies, ethanol was identified as an effective fixation agent because of its low toxicity and ability to preserve tissue...