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Nodule bacteria increase the resistance of host plants to abiotic stress factors; however, the role of the genetic potential of rhizobia in the formation of productive salt-tolerant plant-microbial symbiosis remains underestimated. The aim of the study was to evaluate the pool of genes responsible for the salt tolerance of the alfalfa microsymbiont, Sinorhizobium meliloti, using the DNA microarray technique, phenotype microarray (PM), NGS and NNGS-technologies and genome mining (antismash 5.0). As a result of the ...

Genomes of alfalfa root nodule bacteria, Sinorhizobium meliloti, symbionts of alfalfa are enriched in non-symbiotic (cryptic) plasmids, which gene pool is remained weakly studied. S. meliloti strains are significantly varied in number and size of these plasmids. The goal of the study was to assess the occurrence of phage-related sequences (PRS) on cryptic plasmids. Whole genome sequences of 12 S. meliloti strains native to Caucasian and Kazakhstan centers of alfalfa diversity (NCG and PAG, correspondingly) were st...

Sinorhizobium meliloti are agriculturally valuable species of soil bacteria that form nitrogen-fixing symbiosis with alfalfa plants. Global climate changes lead to an increase of agricultural areas subjected to salinity. Current knowledge about about high-salt stress impact on soil saprophitic root nodulated microsymbionts of legumes is weakly studied and rhizobia gene pool responsible for salt tolerance are fragment and far from clear. An increase of bacteria nonspecific resistance (immune status) to unfavorable ...

Soil saprophytic nitrogen-fixing nodule bacteria (rhizobia) of the Sinorhizobium meliloti species are formed symbiosis with alfalfa and can also be a host of bacteriophages. Rhizobia have a symbiotic and saprophytic life phase?s cycle. Nodules formed by rhizobia on alfalfa roots are destroyed at the end of the growing season and bacteria surviving in nodule threads and did not transformed into bacteroids are released into the soil microbiome. The last is enriched in bacteriophages, which can significantly decrease...

Symbiotic nitrogen fixation is the most environmentally friendly method for the accumulation of biologically bound nitrogen. Nitrogen fixation is carried out by bacteria, including from the class of ?-proteobacteria. Global climate changes lead to an increase of agricultural areas subjected to salinity. Current knowledge about the effect of high-salt stress on nitrogen-fixing symbiotic ?-proteobacteria has the appearance of a puzzle. The genetic mechanisms determining salt tolerance have been studied in the most d...

Sinorhizobium meliloti species are saprophytic soil bacteria able to fix atmospheric nitrogen in symbiosis with host-plants from alfalfa cross inoculated group. These biotechnologically important species of rhizobia are commonly present in various soil environments, while little is known about their phages. The main objective of the research was to isolate and characterize phages (rhizobiophages) of S. meliloti from soil samples collected at the NE Caucasian origin of alfalfa diversity (NEC). Under our particular ...