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CRISPR-Cas adaptive immunity systems of prokaryotes have been identified in 42% of bacteria and 85% of archaea [1] and are actively used in genetic engineering. However, in nitrogen-fixing nodule bacteria required for agriculture, these systems are very poorly characterized. Therefore, in this study, we evaluated the diversity of CRISPR-Cas systems in the genomes of 39 isolates of alfalfa nodule bacteria Sinorhizobium meliloti recovered from soil samples from Kazakhstan and Turkmenistan, which belong to the Centra...

Nodule bacteria increase the resistance of host plants to abiotic stress factors; however, the role of the genetic potential of rhizobia in the formation of productive salt-tolerant plant-microbial symbiosis remains underestimated. The aim of the study was to evaluate the pool of genes responsible for the salt tolerance of the alfalfa microsymbiont, Sinorhizobium meliloti, using the DNA microarray technique, phenotype microarray (PM), NGS and NNGS-technologies and genome mining (antismash 5.0). As a result of the ...

The aim of the study was to characterize the gelling effect of candelilla wax (DW) using different types of cold-pressed vegetable oil (sunflower oil, olive oil, walnut oil, grape seed oil and hemp seed oil). The structuring of the oleogels was achieved by hot incorporation of oil with different percentages of carnauba wax, as follows: 3%, 5% and 11%. Characterization of the oleogels was done by determining the chemical and physical properties of the oleogels, the oil binding capacity and the texture of the oleoge...

The use of natural products for healing purposes has gained great attention worldwide. Increased interest in certain plant products has been achieved mainly due to their ability to limit antibiotic resistance. Also, as the hypothesis that all biological processes at the cellular level are severely affected by the formation of free radicals has been confirmed, it is attempted to highlight some active principles in plants and their use as agents to counteract the oxidative process [1, 2]. This paper aims to demonstr...

Echinacea purpurea is a herbaceous plant that is 80-150 cm tall. [1] The roots of the plant penetrate deep into the soil, they have fibrous ramifications, the rhizome is short. Its leaves are wide, lanceolate or elliptic-oblong, and the flowers are conical, have a violet color. Echinacea species are native to North America and in Europe they are cultivated as medicinal or ornamental plants. [17] The research focused on determining the photosynthetic rate in this species, using variants cultivated both in vivo and ...

The presence of nitrate concentration in drinking water can cause high toxicity to the human body, being considered among the most important pollutant in the environment. Among the many methods available such as biological treatment, electrodialysis, reverse osmosis, adsorption, ion exchange, adsorption is the most used technique due to its low cost and simplistic design. Therefore, the aim of this paper was to study the retention of nitrate anion from high concentration aqueous solutions on a novel magnetic adsor...

Fish oils with a high content of polyunsaturated fatty acids (PUFAs) are gaining increasing interest in the food and nutraceutical industry, due to their important functional properties for human beings- health improvement. The most important PUFAs have at least two double bonds placed on the carbon chain. These include ?-linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahaxaneoic acid (DHA). Microencapsulation is a technique used to protect the bioactive compounds from oxidative damage, to ensure their ...

Sinorhizobium meliloti are agriculturally valuable species of soil bacteria that form nitrogen-fixing symbiosis with alfalfa plants. Global climate changes lead to an increase of agricultural areas subjected to salinity. Current knowledge about about high-salt stress impact on soil saprophitic root nodulated microsymbionts of legumes is weakly studied and rhizobia gene pool responsible for salt tolerance are fragment and far from clear. An increase of bacteria nonspecific resistance (immune status) to unfavorable ...

The aim of this work is to optimize the growth parameters of D. salina by altering nutritional factors, in order to maximize production of antioxidants and lipid fraction by the microalgae strain. For this purpose, the concentrations of different salts in the growth medium were varied. The biomass obtained was analyzed quantitatively and qualitatively in order to determine biomass concentration and bioactive compounds concentrations in respect to the growth medium composition. Pigments were extracted from the drie...

The effectiveness of rhizobia-legume symbiotic interaction is under the genetic control of macro- and microsymbionts. Both symbionts exhibit high level of genome/genome specificity interaction which is reflected by symbiotrophic indices vary significantly. Virulence and plant dry mass increasing are indices widely used in order to reveal well matched rhizobia strain and variety of legume host plant for practice demand. The aim of the research was to study how allelic polymorphism of symbiotically significant genes...

An interest to bacteriocins as natural alternative for antibiotics and chemical preservatives is grow at present. The selection of novel strains producing antimicrobial peptides is in focus. The probiotics are preferable because they are recognized as safe and thereby can be applied in medications, foods, feeds. Moreover, the benefits of probiotics for human and animal health is proved. The growth kinetics of industrial probiotic strains Lactobacillus plantarum B-11007, Lactobacillus salivarius B-2214 ? Lactobacil...

Soil saprophytic nitrogen-fixing nodule bacteria (rhizobia) of the Sinorhizobium meliloti species are formed symbiosis with alfalfa and can also be a host of bacteriophages. Rhizobia have a symbiotic and saprophytic life phase?s cycle. Nodules formed by rhizobia on alfalfa roots are destroyed at the end of the growing season and bacteria surviving in nodule threads and did not transformed into bacteroids are released into the soil microbiome. The last is enriched in bacteriophages, which can significantly decrease...

Currently, CRISPR/Cas systems are actively studied bacterial systems, due to the possibilitpossibility to apply them for targeted editing of eukaryotic genomes. CRISPR/Cas systems are belonged to adaptive immun immune system of bacteria and archaea acting against invading mobile genetic elements (MGEs: bacteriophages and integrative conjugative elements ). Curre Currently CRISPR/Cas systems were detected in genomes of 75% of known archaea and 36 % of bacteria strains . However, there are still quite less knowledge...

Nodule bacteria (rhizobia) forming nitrogen-fixing symbiosis with legumes have a multicomponent genome represented by chromosome and a number of plasmids of different sizes. Genes related to symbiotic activity of Sinorhizobium meliloti are located predominantly on two megaplasmids SMa and SMb (1.35 and 1.68 Mb, correspondingly). Genomes at about 80% of native rhizobia nodulating alfalfa contained cryptic plasmids with sizes varied from 7 up to 450 kb. Functional role of this class of plasmids remained unclear up t...

Currently, one of the priorities for sustainable development is human life getting better with functional foods. Non-dairy matrices are considered as promising alternative for production of foods and beverages with probiotic microbes. The development of these new products appears to be a hot area in which probiotic cereal beverages occupy a special place. The corn, buckwheat, oats, rye, wheat and barley flour was studied. Two-stage enzymatic hydrolysis of the water suspensions of the flour with amylases and protea...

An actual problem of the bread making industry is represented by the final properties of the desired product. This fact requires a well knowledge of the technological process, the properties of the flour used, the proportions and the dough mixing method, factors that contribute to the desirable properties of the final products. This study aims at comparing the two basic methods of dough obtaining ? the direct method and the indirect method, with direct reference to the dough improved with lentil flour in addition ...

An actual problem of the bread making industry is represented by the final properties of the desired product. This fact requires a well knowledge of the technological process, the properties of the flour used, the proportions and the dough mixing method, factors that contribute to the desirable properties of the final products. This study aims at comparing the two basic methods of dough obtaining ? the direct method and the indirect method, with direct reference to the dough improved with lentil flour in addition ...

Waste products of plant origin are valuable secondary raw materials in the process of agricultural wastes utilization into products with high added value. The application of secondary raw materials is complicated due to the high fibrecontent. The aim of this study was to identify strains of microorganisms capable to produce a wide range of exocellulolases in order to reduce the crude fibrein mild conditions without applying expensive commercial cellulases. The following strains were chosen for this experiment: Tri...

Waste products of plant origin are valuable secondary raw materials in the process of agricultural wastes utilization into products with high added value. The application of secondary raw materials is complicated due to the high fibrecontent. The aim of this study was to identify strains of microorganisms capable to produce a wide range of exocellulolases in order to reduce the crude fibrein mild conditions without applying expensive commercial cellulases. The following strains were chosen for this experiment: Tri...

Worldwide, bananas are commonly consumed. Due to this fact large amounts of banana peels waste are generated. Banana peels contain adequate amounts if starch. The aim of this study was to evaluate the quality of vinegar obtained from banana peels in comparison with other types of commercial vinegar. Color, total dry extract, total titratable acidity, specific gravity, total soluble solids, antioxidant capacity and polyphenols content were determined on different types of vinegar. The analyzed samples were three ty...

Worldwide, bananas are commonly consumed. Due to this fact large amounts of banana peels waste are generated. Banana peels contain adequate amounts if starch. The aim of this study was to evaluate the quality of vinegar obtained from banana peels in comparison with other types of commercial vinegar. Color, total dry extract, total titratable acidity, specific gravity, total soluble solids, antioxidant capacity and polyphenols content were determined on different types of vinegar. The analyzed samples were three ty...

Development and consumption of frozen products is an aspect that is strongly related to nowadays consumers. These are considered less nutritious and less healthy than fresh ones due to processing, artificial ingredients and additives used for production. The paper focuses on the influence of frozen storage of bread dough enriched with lentil flour. The addition of lentil flour in different proportions (15% and 30%) was investigated in order to study the physicochemical and organoleptic effect concerning the obtain...

Development and consumption of frozen products is an aspect that is strongly related to nowadays consumers. These are considered less nutritious and less healthy than fresh ones due to processing, artificial ingredients and additives used for production. The paper focuses on the influence of frozen storage of bread dough enriched with lentil flour. The addition of lentil flour in different proportions (15% and 30%) was investigated in order to study the physicochemical and organoleptic effect concerning the obtain...

The study was performed in order to establish the efficacy of the biological product пїЅNemagoldпїЅ in the control of root-knot nematodes (Meloidogyne spp.) on tomato crop under plastic tunnel conditions. Experiment was organized on tomato crop (cultivar пїЅPrekos F1пїЅ) strongly attached by root-knot nematode, located in south-east of Romania (Varasti-Giurgiu), during July-September 2016 period. Treatments were applied by drip irrigation during the second vegetation season. пїЅNemagoldпїЅ product having in compos...

An marinegastropod mollusk or whelk is well-known Rapana venosa with the common name the veined rapa whelk or Asian rapa whelk. It is a species of large predatory sea snail, in the family Muricidae, the rock shells. This large sea snail has become an invasive species in many different localities around the world. In the area of the Romanian seaside is also found Rapana venosa. Bivalvia is a class of marine and freshwater molluscs that have laterally compressed bodies enclosed by a shell consisting of two hinged pa...

In this work, the filamentous fungus Aspergillus niger was tested for its compatibility with different media compositions, other plant growth promoting microorganisms, and plant growth stimulators. As a first step, a medium selection was carried out employing as a criterion the P-solubilizing activity of A. niger. The highest soluble P concentration of 136,92 mg/L and 394,93 mg/L was registered on Potato-Dextrose and Czapek-Dox medium in conditions of submerged and solid-state fermentation processes, respectively....

Natural compounds and products are increasingly demanded by the pharmaceutical and cosmetics industries as safer and user friendly alternatives to synthetic variants. Young wheat plantlets, known as wheatgrass, are a valuable source of minerals, vitamins and chlorophyll pigments and the biosynthesis of such compounds may be influenced by the cultivation conditions. The present study focuses onto the chemical profile, mineral and chlorophyll content of cultivated wheatgrass using two different types of water (sprin...

To increase the efficiency of conversion of plant raw materials, a search for new enzymes is required. The hydrolysis of xylan using industrial xylanases is widely used in the production of bleached cellulose. However, an increase in activity, selectivity and thermostability of enzymes is relevant for modern biotechnology. Recently, the sources of bacteria that have important practical significance are very diverse: from sea depths to volcanoes. But there are also accessible habitats, for example deposits of waste...

These researches were conducted in the greenhouses resort didactics of the faculty of Horticulture and Forestry in Timisoara, using cuttings of Parthenocissus quinquefolia that were purchased from the mother plant from the same location. Four substrates were used for rooting namely sand, peat, perlite, perlite + peat, the cuttings were planted and treated with rooting substance of Atonic and Radistim. During the study was monitoring the rate of rooting of cuttings, aiming at influence of substrates and rhyzogene s...

This paper shows laboratory researches in order to extract arsenic and cadmium from the polluted soils. Research is based on preliminary studies regarding the usage of microorganisms which were selected from the same sampling points of soil assays for decontamination of soil polluted with heavy metals (As and Cd). The soil samples are subjected to the cleaning process with different extraction solutions (9K medium and chemical solutions) stirred 200 rpm for 72 hours at a controlled temperature (32 пїЅC). During th...

In the study presented were used Pandorea jasminoides cuttings, they were planted in three different periods and treated with rooting stimulants. It was followed the rooting rate, depending on the period of the planting cuttings and depending on rooting substance that has been used. The experiment was conducted in a closed greenhouse in the Faculty of Horticulture and Forestry in Timisoara. Considering the three periods of initiation of the process of rooting cuttings, Pandorea jasminoides averages were recorded c...

This work has pursued the accumulation process of aromatic compounds such as esters in three varieties of Romanian native wines from Apoldu de Jos, namely Feteasc? regal?, Feteasc? alb? and Iordana, obtained by fermenting them in a microvinification system, in a bioreactor equipped with temperature sensors for pH and CO2. The pectolytic enzymes Enozym flavors (3g/100 kg) and selected Vinartis ferm yeasts as Saccharomyces cerevisiae strain were used for the alcoholic fermentation. To identify and quantify the ester...