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For increasing the volumes of the grain production and quality it is necessary to restore and extend sowing area, enlarge the yield growth at the expense of intensive technologies of cultivation, effective selection and decrease of losses while harvesting. One of the main reasons of yield shortage is lodging of sowings. This paper compares extent of grain crops lodging. The investigation was carried out using mathematic analysis of variety features of the stem resistance for winter and spring wheat, barley, rye, t...

Matrix metalloproteinases (MMP) belong to the family of zinc metalloprotenases, whose function is related to the metabolism of the extracellular matrix proteins. These enzymes play an important role in the development of physiological processes such as morphogenesis, resorption and tissue remodeling, migration, adhesion, cell differentiation and proliferation, as well as in pathological conditions (rheumatoid arthritis, glomerulonephritis, periodontitis, ulceration of the cornea, etc.). To the first, the most stud...

Alzheimer`s diseases (AD) is the most common age-related dementia, being characterized by accumulation of extracellular amyloid plaques in AD brains. The principal constituent of these plaques are fibrils which are primarily composed of amyloid-? peptides (A?), with 39-42 amino acid residues in length, which are the proteolysis products derived from the amyloid precursor peptide (APP). Several factors, such as oxidative stress, inflammation, metal ions or genetic factors are associated with AD. Even if metal ions ...

Natural dyes gained considerable attractiveness in textile manufacturing based on the negative impact of synthetic dyes on the environment and human health. The aim of the present study was to develop an efficient extractive technology of anthocyanins from Hibiscus sabdariffa, and to comparatively investigate the dyeing of cotton with the natural extract, in the presence and absence of (bio)mordants. The results of extraction processes using eco-friendly solvents and different solvent/solid ratio and extraction ti...

Hazardous household waste (HHW) is a small but the most dangerous fraction of household waste. Different studies estimate their content in household waste from 0.1 to 1%. In Eastern Europe, due to the low efficiency of separate household waste collection, most of the hazardous household waste is mixed with other waste and are mainly disposed in landfills. A thorough analysis of the literature on HHW has been carried out. Statistical data on HHW generation in the Eastern Europe countries (Bulgaria, Czech Republic, ...

The solid waste generated in food industry was considered as a low cost raw material for the extraction of natural antioxidants. Unfortunately, some of these wastes are not dealt with properly and are causing considerable damage to the environment. Agricultural wastes in Romania amount range from 7600 thousand tons a year of which only 1400 thousand tons as animal feed and 1100 thousand tons as organic manure are being utilized. These crop waste results after harvesting in the farm of leaves stem which are charact...

Sinorhizobium meliloti species are saprophytic soil bacteria able to fix atmospheric nitrogen in symbiosis with host-plants from alfalfa cross inoculated group. These biotechnologically important species of rhizobia are commonly present in various soil environments, while little is known about their phages. The main objective of the research was to isolate and characterize phages (rhizobiophages) of S. meliloti from soil samples collected at the NE Caucasian origin of alfalfa diversity (NEC). Under our particular ...

Bacteriorhodopsin (bR) is a red-colored protein found in cell membranes of the organism Halobacterium salinarum, being considered as a light activated proton pump that transports protons across the plasma membrane. Because bacteriorhodopsin is one of the simplest ATP-producing systems known in biology, it has been the subject of intensive investigations. The bR photo-intermediates were seen in the mechanistic model considering that this protein links light energy to the translocation of protons across the bacteria...

Baltic sprats, being one of the primary fishing objects of the Baltic Sea, are primarily used for canned and spiced fish production. Given the ability of sprats to ripen in salt, the current study looks to obtain sprat-based products implementing the technological scheme similar to the production scheme of anchovies in oil and anchovy pastes popular on the European market. As part of the preliminary work, the technochemical composition of the Baltic sprat and that of the European anchovy (Engraulis engasicholus) w...

Neurodegenerative diseases are a group of pathologies that mainly affect neurons of the human brain. Particularly, Alzheimer disease (AD) is a neurodegenerative disorder, being manifested by neuronal and synaptic loss. One of the main pathological aspects of AD is aggregation of amyloid-? (A?) peptide. However, there are some neuropeptides that play an important role in immunity and neuroprotection. In addition, the presence of metal ions and their abnormal accumulation play a key role in this pathology. The inter...

Chicken manure is one of the massively produced wastes in the agriculture, and pyrolysis may be a good way of its treatment. Biochar obtained in the process of pyrolysis may be used as soil fertilizer since it improves soil water and air balance, stabilizes pH, provides plants and soil microbiota with nutrients. In the present study, chicken manure with sawdust was pyrolyzed at 400 ?C (residence time 2 h), and biochar obtained was added to soil in a dose of 30 t ha-1 in a field experiment. Wheat was planted in the...

In view of increasing demand for more efficient use of water resources, technological processes are innovated in textile industry and result in lower environmental impact. One possible option is to clean textile waste water containing industrial dye residues by microbial consortia and biodegradation process. This method of removing potentially toxic substances by microbial biomass is appropriate because other chemicals are not brought into the environment and residual biomass can be easily removed when the biodegr...

One of the directions of the rational use of the secondary raw materials of meat industry, including collagen-containing, is its modification in order to obtain protein products enriched with micronutrients for the further use in food production. The presence of active carboxyl end groups in the collagen molecule and complex spatial structure cause the complexation of this protein with various minor nutrients, thereby promoting the creation of a biologically active composite for the enrichment of meat products. To...

The research regarding some technological links took place in the Oravita Depression, located in south-western Romania, between 2016 and 2018. The records from the Oravita Meteorological Station show that the average annual rainfall in the area is 736 mm, and the average annual temperature is 10°C. The climate of this area falls within the climatic formula c.f.b.x. according to Koppen, namely in the moderate continental temperate climate. The soil type on which the experiments were performed was stagno-gleyic prel...

The compositions of polymer biodegradable films based on polyhydroxybutyrate and chitosan, cross-linked with calcium chloride, have been developed. New materials, which are promising for use in the field of medicine as wound coverings, suture materials and tissue engineering, have been obtained by casting method. The effect of prescription parameters such as the content of plasticizer and calcium chloride on the physic-chemical properties of polymer compositions has been studied. It is shown that by varying the co...

One of the risk factors in skin cancer is unprotected exposure to UV radiation. Melanoma is the deadliest form of skin cancer but also curable if it is diagnosed in the early stages. This study uses a histogram analysis technique to investigate and classify two common skin lesions, nevi and melanoma. The colour feature investigated is the optimal threshold values in RGB space. Also, a boundary limit of the skin lesions is performed using a colour histogram analysis and the optimal threshold determination. A statis...

Enzymatic conversion of lignocellulosic substrates into sugars is an upcoming sphere of biotechnology. Most of the ways, aimed on hydrolysis process improvement are focused at maximizing of raw materials conversion into monosaccharides. Thus, process improvement usually focuses on substrates pretreatment, choosing of the most effective enzyme complexes and control of the hydrolysis process parameters such as increasing the process duration and enzyme dosage, substrate concentration reduction. Kraft woody pulp enzy...

This study was conducted to determine the effects of various levels of dietary protein and short-term starvation periods on compensatory growth, body compositions and organ indices of rainbow trout (Oncorhynchus mykiss) during 46 days. Fish were fed ad libitum twice a day or starved as follows: two control groups, one group was feed daily with extruded pellets containing 41% crude protein (D41) and the other control group was feed daily with commercial pellets containing 50% crude protein (D50); two groups fasted ...

Vegetables are considered a rich source of antioxidants (flavonoids, carotenoids, vitamins), which are frequently added to stop the process of oxidation in processed food systems. The solid waste generated in food industry was considered as a low cost raw material for the extraction of natural antioxidants. The effect of drying techniques (ambient-drying and oven-drying) on the total phenolic contents and antioxidant activity of selected vegetables byproducts (garlic (Allium sativum L.), tomato (Lycopersicon escul...

Acidophilic microorganisms oxidizing ferrous iron and sulfur are widely used in hydrometallurgy to extract different metals including copper, zinc, nickel, cobalt, and uranium from sulfide ores. The activity of microorganisms and screening of microbial strains capable of biooxidation of ferrous iron and sulfur compounds under different conditions, i.e. in the present of different toxicants, which may be present in treated ores, are crucial for the development of biohydrometallurgical technologies. Different factor...

The study presented in this paper is related to the extraction of humic lignite substances and the separation of fulvic acids in order to introduce them into a fertilizing matrix and to evaluate the effect on tomato culture. Fulvic acids have biostimulatory effect on agricultural crops and have significant uses in agriculture. For the characterization of fulvic acids (FA), the total acidity and content of carboxylic groups were determined. The separate fulvic acids were introduced into a matrix containing nutrient...

Plants have been considered as materials for alternative medicine and sources of pharmaceutical drugs. Despite the therapeutic advantages of medicinal plants, some of their constituents may be potentially toxic, mutagenic, carcinogenic, or teratogenic potentials. Therefore, the aim of our study is to determine the cytogenetic and genotoxic potentials of aqueous extract of Plantago lanceolata regarding quality, safety and efficiency. The cytogenetic effects were studied by the calculation of the mitotic index, by t...

Food processing wastes are the end products of the food processing industries which cannot be recycled or used for other purposes. These are the non-product flows of raw materials whose economic values are less than the cost of collection and recovery for reuse; and therefore discard as a waste. These wastes pose increasing disposal and potential severe pollution problems and represent a loss of valuable biomass and nutrients. Onion solid wastes (OSW) were used as raw material to produce polyphenol and extracts wi...

The molecular characterization of Genetic diversity of ten bread wheat (Triticum aestivum L.) varieties was evaluated by studying high molecular weight (HMW) glutenin subunits. Polymorphism of HMW-SG war carried on by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Among genotypes analyzed, twelve polymorphic bands were identified at the three loci Glu-A1, Glu-B1 and Glu-D1. The analysis of HMW-GS reveals a great diversity at the level of the three loci and more particularly on the locus (Gl...

The search for new microorganisms ? sources of thermostable and thermoactive amylolytic enzymes is an important direction for the efficient processing of plant raw materials at high temperatures. A bacterial culture obtained from the thermal spring in the southeast of Iceland was used in the research. The identification of bacterial species by 16S rRNA gene sequencing has shown that the detected bacteria belong to the species Geobacillus kaustophilus. Microorganisms grown on a medium with the following composition...

Due to their huge volumes, toxicity and destruction resistance petroleum and petroleum products are the most important polluters. The source of oil pollution is not only oil extraction processes, but also oil transportation, refining and use. Ecologically safe and effective way to clean the environment off petroleum hydrocarbons is bioremediation. The effectiveness of oil-oxidizing biological products is ultimately determined by the activity of contained hydrocarbon oxidizing bacteria strains. The search, isolatio...

Technological parameters of the introduction of grapes into the in vitro culture have been improved. The use of growth regulators (6-BAP, 2iP, kinetin) contributed to the enhancement of rhizogenesis and an increase in the coefficient of in vitro plant reproduction. New methods for adapting grape plants that were in vitro improved to in vivo conditions at the reproduction stage have been studied. It has been shown that all concentrations of lingo-humate had a positive effect on plants, which in turn contributed to ...

The development of the most efficient technology is extremely important to synchronize work on the laying yielding strawberry parent plants. The main link here should be the material obtained in vitro. The most vulnerable element of in vitro technology is adaptation to non-sterile conditions, when it is very difficult to simultaneously control a large number of factors and when very large plant losses are possible. The fundamentally new method of adapting microplants to hydroponics has been tested and allows us to...

The aim of this study is to evaluate the influence of adulteration agents (glucose, fructose, inverted sugar, and hydrolysed inulin syrup and malt wort) addition into honey on texture parameters (viscosity, hardness, adhesion, springiness, cohesiveness, chewiness and gumminess). For this purpose, 5 honey samples of different botanical origins (acacia, honeydew, polyfloral, sunflower and tilia) has been adulterated with different percentages 0, 5%, 10%, 20%, 30%, 40% and 50% respectively with glucose, fructose, inv...

Water deficit is the major environment factor affecting bread wheat (Triticum aestivum L.) grain quality under Mediterranean climate. Many ecological regions in Algeria are suitable for the production of bread wheat, and therefore the technological quality of this wheat should be improved. The determination of the technological quality of bread wheat (Triticum aestivum L.) requires a strong investment of time and money on behalf of the flour industry. For this reason, a field experiment was carried out over a 1-ye...

The sustainable agriculture requires a series of complex, individual but interrelated and dependent on each other processes that need to be efficiently organized in stages to ensure good overall results. Therefore, the success of a given product is based on a triangle formed by the farmer, the technology and the concept of services and counseling. Services and counseling are based on collected data. Data generated from modern agricultural activities is extracted with the usage of various sensors embedded in agricu...

Ketoprofen is well-known drug due to anti-inflammatory, analgesic and antipyretic properties, and is widely used in the treatment of various acute and chronic inflammatory diseases, including rheumatoid arthritis, osteoarthritis, ankylosing spondylitis as well as menstrual abdominal cramps. Its therapeutic benefits in the prevention of various cancers including colorectal and lung cancers as well as in the treatment of neurodegenerative disorders such as Alzheimer?s disease, Parkinson?s disease have been establish...

The biosynthesis of noble metal nanoparticles using microorganisms (?green synthesis of nanoparticles?) has recently become widespread for medical applications. The characteristics of the resulting nanoparticles depend on the microorganisms used and the process conditions. Screening of a number of methylotrophic bacterial communities of a practical biotechnological value was carried out to determine those ones, which were able to synthesize silver and palladium nanoparticles at high rate and efficiency. In several...

The paper emphasize the correlation among the metals content in different samples of some individual macrophyte algae species with different contribution, as part of residual algae biomass along Romanian Black Sea Coast, that could be used as a potential new biostimulator-regenerator recommended for improving the quality of the vineyard soils from Dobrudja region, Romania. Concentrations and sources of copper, zinc, iron, chromium, manganese, cadmium, lead and aluminium were analyzed. For the metals content determ...

Synbiotics are defined as a composition of probiotics and prebiotics with synergistic reinforcing effects. In practice, only growth stimulation is often took into account when justifying the effectiveness of a synbiotic composition. However, the effect of prebiotics to the interaction of probiotic and pathogenic bacteria during co-culture fermentation was examined in a range of researches. The influence of commercial prebiotics on the ability of probiotics (bifidobacteria and lactobacilli) to suppress the growth o...

This study aimed to identify a rational treatment for soil and determine the efficiency of the use of chelated liquid microfertilizers on the yield of spring hard wheat crops in chernozemic soils of the Samara Region of Russia. The study was conducted as a two-factor field experiment using a recognized variety of spring hard wheat:?Bezenchukskaya stepnaya?. The soil of the experimental field was typical medium-deep heavy loamy chernozem. Data on some agrophysical properties of the soil, weed infestation of crops, ...

The aim of the work was to optimize the process of obtaining multicomponent protein compositions with high biological value and higher functional properties than the original vegetable protein products. Initially, studies were carried out to obtain a biocomposite composition of dry wheat gluten (DWG) - pea protein, DWG-potato protein, potato protein-oat protein. Composites are enriched with all three limiting amino acids: lysine, threonine, sulfur-containing amino acids. For each of the essential amino acids, amin...

Poultry meat and table eggs are known to be a popular food items all over the world. The nutritional value of poultry white meat is of great value over the red meat. It contains considerable amount of protein, minerals, vitamins, and fat. In order to ensure a good quality of poultry products, the flock should be a good health and free from infectious agents. Antibiotics were widely used to prevent diseases in poultry flocks. However, using antibiotics for long time may lead to microbes that are resistant to drugs....

Morphogenesis of structural elements of secondary xylem is associated with the biosynthesis of a massive secondary cell wall consisting of three layers, differing in mass, composition and structural organization. Cellulosic microfibrils are basic structural elements fixing cell walls. The participation of cellulases in cellulose biosynthesis is shown. Overexpression of cellulases (EGase) increased cell expansion and elongation and resulted in an increase in plant growth and biomass production. However, the precise...

The purpose of the study was the laboratory testing of the pendant seeder-spreader (production of China) as part of the universal unmanned aircraft system "OSA HEXA" based on UAV "OSA" (developer and producer was Bozon LLC, Moscow), in connection with which we solved the following tasks: 1) determination and assessment of performance indicators of the functioning of spreading device; 2) selection of the best modes of application of the test object; 3) selection of significant factors affecting the quality indicato...

In this study we performed a comparative analysis of the flavonoid compounds found in the composition of some species belonging to the Cucurbitaceae family, namely: cucumber (Cucumis sativus), bitter melor (Momordica charantia), zucchini or courgette (Cucurbita pepo) and horned melon (Cucumis metuliferus). The flavonoids in these species were identified by high performance liquid chromatography. The Cucumis metuliferus plant extract had the highest content in phenolic compounds (14.424 mg/L), while the Cucumis sat...

Consumption of muffins has become very popular around the world. Various ingredients have been used to obtain these muffins and different recipes can be found in the literature. In this study three different samples (S) of muffins (S1- with wheat flour, S2 ? with wheat flour: almond flour (1:1) and S3 ? with almond flour) were prepared and investigated. Muffins also contain, besides flour: eggs, coconut oil, sugar, nuts, cherries and orange juice in the same proportions for all samples. The composition is dosed in...

Model for a change of the biosystems states on the grounds of Markov chains is offered in the work. One of the main problems of management of the biosystems states is the study of the mechanisms, which determine dynamic features of the processes, taking place in the natural environment. Therefore, there is a necessity in the development of special methods, allowing to study dynamic properties of natural objects without subjecting them to ruination. Naturally, a mathematical modeling method may satisfy this demand ...

New functional foods produced by fermentation of pretreated with amylolytic and proteolytic enzymes cereal raw materials suspensions by probiotics are presented. Whole meal wheat flour comprising organic compounds and microelements was used as source of nutrients. Duozyme (Novozymes) amylases (1% by weight of the substrate) and Protex 40E (Genencor) proteases (2% of the protein content in flour) were applied for hydrolysis. The starter culture was one of the gram-positive probiotic lactic acid bacteria Lactobacill...

The increasing amount of fiber on wheat flour brings some deleterious effects on dough structure, such as the increasing of dough stickiness and fermentation tolerance decreasing. Also it has a negative influence against other properties of dough such as handling and rising properties. Fiber addition usually causes a decrease in bread volume and breadcrumb loses its elasticity. So, we had investigated if the xylanase addition could counteract those deleterious effects. Positive action of xylanase in dough could be...

Frozen fish is the primary raw material used in the industrial preparation of semi-finished ready to eat fish-based products. Ready to eat fish product manufacturers receive frozen fish raw materials from suppliers and defrost it before processing. Such raw materials are commodities; frozen fish are easily transported around the world, can be stored for a long time, and used for further processing as required, evenly distributing the company?s production capacity throughout the year and, thus, optimising costs. No...

This paper presents the stages of embryonic and postembryonic development in Polyodon spathula (Polyodontidae) under the conditions of the acclimatization area at Fish Culture Research and Development Station Nucet, Dambovita county, Romania. Embryonic development at the paddlefish is carried out in the temperature range of 14-18o C with an optimum of 16o C. For the induction of maturation and ovulation, the Nerestin 5A hormone was used. The size of the eggs is between 2.7-3 mm and light gray to gray-black in colo...

A vital role in detecting eye disorders is played by the analysis of the human retina using medical imaging methods. The main objective of this study is to improve the detection of retinal blood vessels by ameliorating the overall quality of non-uniform illumination conditions, removing image artifacts, connecting the broken vessels, and generating accurate segmented retinal vascular systems. A three-stage edge detection framework is proposed. First, image denoising, contrast enhancement, stretched decorrelation a...

Neurodegenerative diseases such as Alzheimer's and Parkinson's affect an increasing number of people. Their etiology is related to the formation of fibrils and protein aggregates. Therefore, new therapeutic strategies and compounds are required to prevent aggregation of ?-amyloid (A?) peptides. There are some neuropeptides that play an important role in the immune regulation, as well as in neuroprotection; therefore, they might be possible candidates for future drug development. Consequently, our experiments were ...

Industrially defatted non-dehulled sunflower meal (SFM) with a protein content 22%, fibre 38% and cellulose 23% was separated into several fractions: protein fraction and lignocellulosic fraction, then they can be used for bioconversion into value added products. The protein concentrate (PC) contained 83% of protein. Hydrolysis of this concentrate by the commercial proteases, such as Proteinase T, Protex 7L, Protex 40E (Danisco Genecor, Denmark) and Pancreatin (Biosynthesis, the Russian Federation) can transform t...