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Industry is constantly forced to look for new ways to make production processes environmentally safe and products more competitive. In this regard, microbiological enzyme preparations increasingly replace conventional chemical catalysts in a number of industrial processes. Such preparations, in addition to environmental friendliness and high activity, have a number of advantages over enzyme preparations of vegetable and animal origin. With the aim of creating new recombinant lipase, we analyzed data on the activit...

Industry is constantly forced to look for new ways to make production processes environmentally safe and products more competitive. In this regard, microbiological enzyme preparations increasingly replace conventional chemical catalysts in a number of industrial processes. Such preparations, in addition to environmental friendliness and high activity, have a number of advantages over enzyme preparations of vegetable and animal origin. With the aim of creating new recombinant lipase, we analyzed data on the activit...

In the work we propose the model of cellular automata for predator-prey system, which is based on the system of lattice differential equations with delay. The model is based on biological assumptions for arbitrary cell. Each cell can assume a value of state in some discrete scales, which mean density of predator or prey population that the cell contain, respectively. We have some constant birthrate for prey population. Preys are neutralized by predators with some probability rate. Prey population tends to some car...

In the work we propose the model of cellular automata for predator-prey system, which is based on the system of lattice differential equations with delay. The model is based on biological assumptions for arbitrary cell. Each cell can assume a value of state in some discrete scales, which mean density of predator or prey population that the cell contain, respectively. We have some constant birthrate for prey population. Preys are neutralized by predators with some probability rate. Prey population tends to some car...

In our study, we optimized two methods of enzyme activity estimations characterizing C and P cycles (?-1,4-glucosidase and acid phosphatase) for agricultural soil with 2.629% of total C and 0.062 % of total N sampled in Tatarstan (Russia). Concentration of the substrates (4-MUF-?-d-glucopyranoside, 4-MUF-phosphate) in the range from 20 to 900 ?mol g-1 soil was used, four levels of buffer pH (3, 6, 8, 11) were compared, and three different dilutions of soil suspension (1%, 2%, 10% w/v) were investigated. Besides, t...

In our study, we optimized two methods of enzyme activity estimations characterizing C and P cycles (?-1,4-glucosidase and acid phosphatase) for agricultural soil with 2.629% of total C and 0.062 % of total N sampled in Tatarstan (Russia). Concentration of the substrates (4-MUF-?-d-glucopyranoside, 4-MUF-phosphate) in the range from 20 to 900 ?mol g-1 soil was used, four levels of buffer pH (3, 6, 8, 11) were compared, and three different dilutions of soil suspension (1%, 2%, 10% w/v) were investigated. Besides, t...

Nosocomial infections have an important place in human pathology, becoming a major public health problem due to the high frequency of multiresistant strains, with afferent consequences. In the Intensive Care Units (ICU), nosocomial infections represent a constant risk due to a cumulus of factors that increase susceptibility to infections, respectively: decreased immunity due to underlying pathology, patient exposure to a series of invasive manoeuvres, poor nutritional status etc. The purpose of this study was to e...

Nosocomial infections have an important place in human pathology, becoming a major public health problem due to the high frequency of multiresistant strains, with afferent consequences. In the Intensive Care Units (ICU), nosocomial infections represent a constant risk due to a cumulus of factors that increase susceptibility to infections, respectively: decreased immunity due to underlying pathology, patient exposure to a series of invasive manoeuvres, poor nutritional status etc. The purpose of this study was to e...

The article deals with the stages of obtaining collagen hydrolyzate by means of biomodification of cattle rumen, as well as the method of its canning by freeze drying. The preparation of cattle rumen hydrolyzate (RHF) was carried out as follows: the scar was pre-rinsed in running tap water for 20 minutes to remove contaminants and mucus, scraping off muscle and adipose tissue; after the run-off of water (15?20 min), the raw material was ground on a top with a diameter of holes of the scoring grid of 2?3 mm. The gr...

The article deals with the stages of obtaining collagen hydrolyzate by means of biomodification of cattle rumen, as well as the method of its canning by freeze drying. The preparation of cattle rumen hydrolyzate (RHF) was carried out as follows: the scar was pre-rinsed in running tap water for 20 minutes to remove contaminants and mucus, scraping off muscle and adipose tissue; after the run-off of water (15?20 min), the raw material was ground on a top with a diameter of holes of the scoring grid of 2?3 mm. The gr...

In this paper we used the research done in a previous laboratory study in which we optimized the addition of seabuckthorn powder, rosehip powder and grape seed extract to the classic yogurt formulation. Yogurt with the best physicochemical and sensory properties was obtained by adding 1.5% rosehip powder and 0.5% grape seed extract, respectively 1.75% seabuckthorn powder and 0.25% grape seed extract to its manufacturing formulation. Prior to inoculation, the ingredients mixture was pasteurized at 85В°C for 30 minu...

In this paper we used the research done in a previous laboratory study in which we optimized the addition of seabuckthorn powder, rosehip powder and grape seed extract to the classic yogurt formulation. Yogurt with the best physicochemical and sensory properties was obtained by adding 1.5% rosehip powder and 0.5% grape seed extract, respectively 1.75% seabuckthorn powder and 0.25% grape seed extract to its manufacturing formulation. Prior to inoculation, the ingredients mixture was pasteurized at 85В°C for 30 minu...

The paper deals with the possibilities of metal recovery from sediment from sludge disposal site after mining activity from locality Zlate hory. The locality is protected from mineralized water, which represents a potential threat for groundwater. This work deals with the use of bacteria Acidithiobacillus ferrooxidans to bioleach polymetallic ores (Cu, Zn, and Fe) from these locality. It is a simple and effective method to recover metals from mineral raw materials, where conventional methods cannot be applied. As ...

The paper deals with the possibilities of metal recovery from sediment from sludge disposal site after mining activity from locality Zlate hory. The locality is protected from mineralized water, which represents a potential threat for groundwater. This work deals with the use of bacteria Acidithiobacillus ferrooxidans to bioleach polymetallic ores (Cu, Zn, and Fe) from these locality. It is a simple and effective method to recover metals from mineral raw materials, where conventional methods cannot be applied. As ...

The work summarizes the results of research into fundamentals of design and application of slow-release and ecologically safe forms of agrochemicals used for protecting plants from pathogens and weeds. The potential of degradable polyester poly(3-hydroxybutyrate) [P(3HB)] for designing long-term formulations of fungicides (tebuconazole) and herbicides (metribuzin) is described. Experimental forms of agricultural preparations were compared with the commercial fungicide Raxil Ultra containing tebuconazole and Sencor...

The work summarizes the results of research into fundamentals of design and application of slow-release and ecologically safe forms of agrochemicals used for protecting plants from pathogens and weeds. The potential of degradable polyester poly(3-hydroxybutyrate) [P(3HB)] for designing long-term formulations of fungicides (tebuconazole) and herbicides (metribuzin) is described. Experimental forms of agricultural preparations were compared with the commercial fungicide Raxil Ultra containing tebuconazole and Sencor...

The main succulent fodder for cattle in the winter-stall period is silage. For ensiling, amaranth was used - a promising high protein fodder crop, capable of satisfying the animals needs in nutrients. The main factor determining the timing of amaranth harvesting is the sum of the active temperatures (climatic conditions), which, when cultivated on silage, must be at least 1000В°C. Ensiling was performed at different stages of vegetation: in the phase of the beginning of flowering, milk and milk-wax ripeness of see...

The main succulent fodder for cattle in the winter-stall period is silage. For ensiling, amaranth was used - a promising high protein fodder crop, capable of satisfying the animals needs in nutrients. The main factor determining the timing of amaranth harvesting is the sum of the active temperatures (climatic conditions), which, when cultivated on silage, must be at least 1000В°C. Ensiling was performed at different stages of vegetation: in the phase of the beginning of flowering, milk and milk-wax ripeness of see...

Considering the current context in which all producers are trying to optimise processes and resources, making improvements to traditional practices is of great interest in order to fulfil customer expectations for high quality and safe products. Alternatives to enhance and complement traditional winemaking practices are emerging technologies such as ultrasound addressed towards reducing the fermentation time and increasing the extraction of phenolic compounds, controlling a variety of wine spoilage organisms, wine...

Considering the current context in which all producers are trying to optimise processes and resources, making improvements to traditional practices is of great interest in order to fulfil customer expectations for high quality and safe products. Alternatives to enhance and complement traditional winemaking practices are emerging technologies such as ultrasound addressed towards reducing the fermentation time and increasing the extraction of phenolic compounds, controlling a variety of wine spoilage organisms, wine...

The work described the obtaining and studying the properties of alginate-PLGA matrixes containing agglomerates of hydroxyapatite nanoparticles. Hydroxyapatite (HAP) nanoparticles were obtained by controlled deposition in the presence of a stabilizer - sodium citrate. Two types of nanoparticle lamellar (30 to 50 nm in size) and rod-shaped (150 to 200 nm in length) were used in the work. The size and morphology of the hydroxyapatite nanoparticles were studied using a transmission electron microscope. The composition...

The work described the obtaining and studying the properties of alginate-PLGA matrixes containing agglomerates of hydroxyapatite nanoparticles. Hydroxyapatite (HAP) nanoparticles were obtained by controlled deposition in the presence of a stabilizer - sodium citrate. Two types of nanoparticle lamellar (30 to 50 nm in size) and rod-shaped (150 to 200 nm in length) were used in the work. The size and morphology of the hydroxyapatite nanoparticles were studied using a transmission electron microscope. The composition...

The possibility of regeneration of plants from somatic and genetic cells open the way of in vitro storage of the genotypes. The utilisation of such a method has a lot of advantages as: relatively reduced space for preservation of a large number of multiplicated clonal plants, the plants are preserved free of pathogens and viruses, in special storage conditions, the plants don?t need special treatments during preservation, the material has an adequate form for establishing nuclear stocks which make possible rapid p...

The possibility of regeneration of plants from somatic and genetic cells open the way of in vitro storage of the genotypes. The utilisation of such a method has a lot of advantages as: relatively reduced space for preservation of a large number of multiplicated clonal plants, the plants are preserved free of pathogens and viruses, in special storage conditions, the plants don?t need special treatments during preservation, the material has an adequate form for establishing nuclear stocks which make possible rapid p...

One of the ways to maintain the normal gastrointestinal tract microflora of poultry is to use prebiotic supplements in their feed. Among the prebiotics used in poultry farming, there are additives based on mannooligosaccharides from the cell walls of the yeast Saccharomyces cerevisiae. There are no similar domestic prebiotic drugs available, which determines the necessity and relevance of their development. For the production of mannose-containing hydrolysates, there was studied the process of hydrolysis of mannan...

One of the ways to maintain the normal gastrointestinal tract microflora of poultry is to use prebiotic supplements in their feed. Among the prebiotics used in poultry farming, there are additives based on mannooligosaccharides from the cell walls of the yeast Saccharomyces cerevisiae. There are no similar domestic prebiotic drugs available, which determines the necessity and relevance of their development. For the production of mannose-containing hydrolysates, there was studied the process of hydrolysis of mannan...

The aim of this study was to analyze the bread quality prepared from a wheat flour of a 1250 type in which four levels of magnesium ions (0, 100, 150 and 200 mg/100g) from lactate salt were incorporated. In order to determine the bread quality the physical, crumb microstructure, textural, colour and sensory characteristics were analyzed. Bread quality characteristics loaf volume, porosity and elasticity slightly increase with the increase level of magnesium ions addition compared to the control sample. The best br...

The aim of this study was to analyze the bread quality prepared from a wheat flour of a 1250 type in which four levels of magnesium ions (0, 100, 150 and 200 mg/100g) from lactate salt were incorporated. In order to determine the bread quality the physical, crumb microstructure, textural, colour and sensory characteristics were analyzed. Bread quality characteristics loaf volume, porosity and elasticity slightly increase with the increase level of magnesium ions addition compared to the control sample. The best br...

Biotechnology represents unique applications of science that can be used to the development of crops with improved nutritional quality, resistance to pests and diseases and reduced cost of production. Agricultural biotechnology can help solve the global food crisis and make a positive impact on world hunger. Crops improved through biotechnology are producing higher yields and of quality. In this study, 10 DH lines derived from Triticum x Zea crosses and mutagenesis between two modern winter wheat genotypes (Izvor ...

Biotechnology represents unique applications of science that can be used to the development of crops with improved nutritional quality, resistance to pests and diseases and reduced cost of production. Agricultural biotechnology can help solve the global food crisis and make a positive impact on world hunger. Crops improved through biotechnology are producing higher yields and of quality. In this study, 10 DH lines derived from Triticum x Zea crosses and mutagenesis between two modern winter wheat genotypes (Izvor ...

Worldwide, bananas are commonly consumed. Due to this fact large amounts of banana peels waste are generated. Banana peels contain adequate amounts if starch. The aim of this study was to evaluate the quality of vinegar obtained from banana peels in comparison with other types of commercial vinegar. Color, total dry extract, total titratable acidity, specific gravity, total soluble solids, antioxidant capacity and polyphenols content were determined on different types of vinegar. The analyzed samples were three ty...

Worldwide, bananas are commonly consumed. Due to this fact large amounts of banana peels waste are generated. Banana peels contain adequate amounts if starch. The aim of this study was to evaluate the quality of vinegar obtained from banana peels in comparison with other types of commercial vinegar. Color, total dry extract, total titratable acidity, specific gravity, total soluble solids, antioxidant capacity and polyphenols content were determined on different types of vinegar. The analyzed samples were three ty...

In this paper are presented some researches concerning the recycling process of Co-Cr powders that is used by the sintering process DMLS. Direct Metal Laser Sintering process is an additive laser manufacturing technology and is used in different domains like tooling, aerospace, medicine and auto. In the tooling domain, Co-Cr alloy powder is sintered by DMLS process, using a Phenix Systems machine, for obtain different mold prototypes. Co-Cr alloy powder presents good mechanical and corrosion properties. The SEM an...

In this paper are presented some researches concerning the recycling process of Co-Cr powders that is used by the sintering process DMLS. Direct Metal Laser Sintering process is an additive laser manufacturing technology and is used in different domains like tooling, aerospace, medicine and auto. In the tooling domain, Co-Cr alloy powder is sintered by DMLS process, using a Phenix Systems machine, for obtain different mold prototypes. Co-Cr alloy powder presents good mechanical and corrosion properties. The SEM an...

Use of fish meals or oils in the poultry diets has made it possible to increase the level of long chain n-3 fatty acids in meat. When meat is enriched with polyunsaturated fatty acids (PUFA), all vegatble fat sources are less effective than marine fats. This effect results due to the content of n-3 fatty acids (FA) because marine fats or oils are composed of eicosapentanoic acid (EPA) and dosahexaenoic acid in generally a higher proportion whereas vegatable oils contain linolenic acid (LNA) whose conversion to lon...

Use of fish meals or oils in the poultry diets has made it possible to increase the level of long chain n-3 fatty acids in meat. When meat is enriched with polyunsaturated fatty acids (PUFA), all vegatble fat sources are less effective than marine fats. This effect results due to the content of n-3 fatty acids (FA) because marine fats or oils are composed of eicosapentanoic acid (EPA) and dosahexaenoic acid in generally a higher proportion whereas vegatable oils contain linolenic acid (LNA) whose conversion to lon...

In the present study are presented the causes and consequences of a bacterial disease signaled to the silver carp farmed in polyculture with Cyprinids and other fish species in ponds. In the spring of 2017, the presence of a bacterial disease of the species Hypophthalmichthys molitrix cultured within the Fish Culture Research and Development Station Nucet, Romania, was first reported. The illness caused the weakening of the fish body (Fulton's coefficient = 1,6 compared to 1,9 for healthy fish) and a slight anemia...

In the present study are presented the causes and consequences of a bacterial disease signaled to the silver carp farmed in polyculture with Cyprinids and other fish species in ponds. In the spring of 2017, the presence of a bacterial disease of the species Hypophthalmichthys molitrix cultured within the Fish Culture Research and Development Station Nucet, Romania, was first reported. The illness caused the weakening of the fish body (Fulton's coefficient = 1,6 compared to 1,9 for healthy fish) and a slight anemia...

The paper presents the results obtained in the artificial reproduction of carp breeds (Cyprinus carpio): Frasinet, Ineu and Ropsa between 2015 and 2017. Carp used in artificial breeding experiments (body weight 4,5 - 8,0 kg / ex., 4-7 years old) were raised in the ponds within the experimental base of the Fish Culture Research and Development Station Nucet, Romania. For the induction of maturation and ovulation the carp pituitary extract (CPE) and Nerestin 6A were used. The carp pituitary extract (CPE) was given i...

The paper presents the results obtained in the artificial reproduction of carp breeds (Cyprinus carpio): Frasinet, Ineu and Ropsa between 2015 and 2017. Carp used in artificial breeding experiments (body weight 4,5 - 8,0 kg / ex., 4-7 years old) were raised in the ponds within the experimental base of the Fish Culture Research and Development Station Nucet, Romania. For the induction of maturation and ovulation the carp pituitary extract (CPE) and Nerestin 6A were used. The carp pituitary extract (CPE) was given i...

It often happens that wheat flour does not have the proper physical, biochemical and technological properties for bakery, biscuits, pasta, etc. or to be nutritionally inappropriate. This occurs when the biochemical composition is unbalanced. Because the structure and quality of the bread is based on the gluten skeleton, the variation in gluten content will obviously influence the quality of the bread. Also, gliadin / glutenin gluten ratio, which influences dough elasticity, extensibility and flour ability to retai...

It often happens that wheat flour does not have the proper physical, biochemical and technological properties for bakery, biscuits, pasta, etc. or to be nutritionally inappropriate. This occurs when the biochemical composition is unbalanced. Because the structure and quality of the bread is based on the gluten skeleton, the variation in gluten content will obviously influence the quality of the bread. Also, gliadin / glutenin gluten ratio, which influences dough elasticity, extensibility and flour ability to retai...

The paper presents the results of the experiments conducted to study the effect of stimulating maturation and ovulation with different types of hormones on the main parameters characterizing the artificial reproduction process of Silurus glanis L. European catfish used in artificial breeding experiments (body weight = 4.0 - 10.0 kg / ex., age 5-8 years) were raised in ponds within the experimental base of the Fish Culture Research and Development Station Nucet, Romania. For induction of maturation and ovulation, c...

The paper presents the results of the experiments conducted to study the effect of stimulating maturation and ovulation with different types of hormones on the main parameters characterizing the artificial reproduction process of Silurus glanis L. European catfish used in artificial breeding experiments (body weight = 4.0 - 10.0 kg / ex., age 5-8 years) were raised in ponds within the experimental base of the Fish Culture Research and Development Station Nucet, Romania. For induction of maturation and ovulation, c...

The paper presents elements of great interest of some researches referring to strategies and processes to reduce the level of alcohol in wine. In recent years, various methods of reducing the alcoholic concentration of wines have been developed, methods like improved technologies of grapes growing, and also developing new ways of removing alcohol from wine. When selecting the method of correcting the alcohol level there must be analyzed various factors such as: the type of wine, the amount of wine produced, the co...

The paper presents elements of great interest of some researches referring to strategies and processes to reduce the level of alcohol in wine. In recent years, various methods of reducing the alcoholic concentration of wines have been developed, methods like improved technologies of grapes growing, and also developing new ways of removing alcohol from wine. When selecting the method of correcting the alcohol level there must be analyzed various factors such as: the type of wine, the amount of wine produced, the co...

Klebsiella pneumoniae and Acinetobacter spp. strains are opportunistic pathogens frequently involved in the etiology of nosocomial infections in immunocompromised patients. A major factor amplifying the risk of these infections is prolonged period of hospitalised patients, especially in intensive care units. Statistically, the strains belonging to the Enterobacteriaceae family and those of the Acinetobacter spp. are ranked the first and respectively, the second among Gram negative bacilli, whose incidence has incr...

Klebsiella pneumoniae and Acinetobacter spp. strains are opportunistic pathogens frequently involved in the etiology of nosocomial infections in immunocompromised patients. A major factor amplifying the risk of these infections is prolonged period of hospitalised patients, especially in intensive care units. Statistically, the strains belonging to the Enterobacteriaceae family and those of the Acinetobacter spp. are ranked the first and respectively, the second among Gram negative bacilli, whose incidence has incr...

Lonicera japonica is a climbing plant which can grow up to 10 m in length; the young stems are often hairy, while mature stems display a brownish bark and are empty on the inside, the leafs are oval and long, the flowers are known for their appealing perfume, white and yellow in colour, and the fruit is small and black. It is a persistent climbing plant, which offers an impressive bouquet of colour, flowers and an inviting perfume, which made it famous in our country as well as abroad. During the study, two rootin...

Lonicera japonica is a climbing plant which can grow up to 10 m in length; the young stems are often hairy, while mature stems display a brownish bark and are empty on the inside, the leafs are oval and long, the flowers are known for their appealing perfume, white and yellow in colour, and the fruit is small and black. It is a persistent climbing plant, which offers an impressive bouquet of colour, flowers and an inviting perfume, which made it famous in our country as well as abroad. During the study, two rootin...